Objective:To compare the circular pathological changes of chronic hepatitis B (CHB) patients according to the tongue diagnosis.Methods:Totally 41 CHB patients with typical white tongue coating (WTC)or yellow tongue coating (YTC) were enrolled and 14 healthy volunteers with normal tongue manifestation served as controls.The mRNA expression of peripheral leukocytes was detected by GeneChips,and 9 genes were randomly selected for expression validation.Circular metabolites were detected by gas chromatography-mass spectrometry.Biological information was analyzed based on ingenuity pathways analysis or metabolomics database and the integrated networks were constructed by ClueGO.Results:A total of 945 and 716 differentially expressed genes were found in patients with WTC and YTC relative to healthy volunteers respectively.The biological information analysis indicated that CHB patients had obviously increased functions in cell death,apoptosis and necrosis (Z-score ≥2,P＜0.05) and decreased activation in T lymphocytes (Z-score ≤-2,P＜0.05),regardless of the tongue manifestation.Compared to patients with WTC,the YTC patients were predicted to be more active in functions related to virus replication (Z-score ≥2,P＜0.05),and the content of circular fatty acids,such as oleic acid (P=0.098) and lauric acid (P=0.035),and citric acid cycle-related metabolites were higher in the YTC patients (P＜0.1).The integrated analysis based on differential genes and metabolites indicated that the most difference in the biological function network between the WTC and YTC patients was tumor necrosis factor receptor associated factor 6 mediated-nuclear factor kappa-B activation process.Conclusions:CHB patients with YTC had more severe inflammation and fatty acids metabolism aberrant than patients with WTC.The results facilitate the modern pathological annotation of Chinese medicine tongue diagnosis theory and provide a reference for the interpretation of pharmacological mechanisms of Chinese medicine treatment.

TANG Yi-shuang;GUO Jian-chun;XU Lin;ZHANG Xiao-na;SHEN Xiao-ping;HAI Ya-mei;MAO Yu-feng;HU Yi-yang;ZHAO Yu

Key Laboratory of Liver and Kidney Diseases,Institute of Liver Diseases,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 201203 ,China;Department of Integrated Chinese and Western Medicine,Hangzhou Xixi Hospital,Hangzhou 310023 ,China;National Engineering Center for Biochip at Shanghai,Shanghai 201203 ,China;Institute of Clinical Pharmacology,Shuguang Hospital affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 201203 ,China;Shanghai Key Laboratory of Traditional Chinese Clinical Medicine,Shanghai 201203 ,China

中国结合医学杂志（英文版）

[1]TANG Yi-shuang;GUO Jian-chun;XU Lin;ZHANG Xiao-na;SHEN Xiao-ping;HAI Ya-mei;MAO Yu-feng;HU Yi-yang;ZHAO Yu-.Pathological Change of Chronic Hepatitis B Patients with Different Tongue Coatings by Circular Multi-omics Integrated Analysis)[J].中国结合医学杂志（英文版）,2022(01):28-35

YTC,GeneChips,ingenuity

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