Insect fecundity is a quantitative phenotype strongly affected by genotypes and the environment.However,interactions between genotypes and environmental factors in modulating insect fecundity remain largely unknown.This study investigated the im-pact of population density on the fecundity of Nilaparvata lugens(brown planthopper;BPH)carrying homozygous high-(HFG)or low-(LFG)fecundity homozygous geno-types.Under low population densities,the fecundity and population growth rate of both genotypes showed similar increasing trends across generations,while the trends between HFG and LFG under high population densities were opposite.Through a combination of temporal analysis and weighted gene co-expression network analyses on RNA-seq data of HFG and LFG under low and high population densities in the 1st,3rd,and 5th gener-ations,we identified 2 gene modules that were associated with these density-dependent progenitive phenotypes.Four pathways related to the neural system were simultaneously enriched by the 2 gene modules.Furthermore,Nipale,which encodes a tyrosine hydroxy-lase,was identified as a key gene.The RNA interference of this gene and manipulation of its downstream product dopamine significantly affected the basic and density-dependent progenitive phenotypes of BPH.These findings indicated that dopamine biosynthesis is the key regulatory factor that determines fecundity in response to density changes in dif-ferent BPH genotypes.Thus,this study provides insights into the interaction of a typical environmental factor and insect genotype during the process of population regulation.

Kai Liu;Longyu Yuan;Lei Yue;Weiwen Chen;Kui Kang;Jun Lv;Wenqing Zhang;Rui Pang

State Key Laboratory of Biocontrol,School of Life Sciences,Sun Yat-sen University,Guangzhou,China;Innovative Institute for Plant Health,College of Agriculture and Biology,Zhongkai University of Agriculture and Engineering,Guangzhou,China;Guangdong Provincial Key Laboratory of Microbial Safety and Health,State Key Laboratory of Applied Microbiology Southern China,Institute of Microbiology,Guang-dong Academy of Sciences,Guangzhou,China

中国昆虫科学（英文版）

[1]Kai Liu;Longyu Yuan;Lei Yue;Weiwen Chen;Kui Kang;Jun Lv;Wenqing Zhang;Rui Pang-.Population density modulates insect progenitive plasticity through the regulation of dopamine biosynthesis)[J].中国昆虫科学（英文版）,2022(06):1773-1789

progenitive,HFG,Nipale

Population,density,modulates,insect,plasticity,through,regulation,dopamine,biosynthesis,Insect,fecundity,quantitative,strongly,affected,by,genotypes,However,interactions,between,environmental,factors,modulating,remain,largely,unknown,This,study,investigated,pact,population,Nilaparvata,lugens,brown,planthopper,BPH,carrying,homozygous,high,low,LFG,Under,densities,growth,rate,both,showed,similar,increasing,trends,across,generations,while,under,were,opposite,Through,combination,temporal,analysis,weighted,expression,network,analyses,seq,data,1st,3rd,5th,identified,modules,that,associated,these,dependent,phenotypes,Four,pathways,related,neural,system,simultaneously,enriched,Furthermore,which,encodes,tyrosine,hydroxy,lase,was,key,interference,this,manipulation,its,downstream,product,significantly,basic,These,findings,indicated,regulatory,determines,response,changes,dif,ferent,Thus,provides,insights,into,typical,during,process

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