典型文献
CRISPR-mediated mutagenesis of the odorant receptor co-receptor(Orco)gene disrupts olfaction-mediated behaviors in Bactrocera dorsalis
文献摘要：
Olfaction plays an essential role in insect behavior such as host location,foraging,mating,and oviposition.The odorant receptor co-receptor(Orco)is an oblig-atory odorant receptor and indispensable in odor perception.Here,we characterized the Orco gene from the oriental fruit fly,Bactrocera dorsalis(Hendel),a notorious agricul-ture pest.The olfactory deficiency mutants were generated by editing the BdorOrco gene using the CRISPR/Cas9 system.Electroantennograms(EAG)and olfactory preference as-says confirmed that BdorOrco-/-mutant flies had reduced perception of methyl eugenol,β-caryophyllene,and ethyl acetate.Oviposition bioassays showed that the eggs laid by BdorOrco-/-females mediated by benzothiazole and l-octen-3-ol were significantly de-creased.In addition,BdorOrco-/-mutant flies took a significantly longer time to locate the food source compared with wild type(WT)flies.Altogether,our data indicated that Orco is essential for multiple physiological processes in B.dorsalis,and it expands our understanding of the function of insect Orco.
文献关键词：
中图分类号：
作者姓名：
Li Xu;Hong-Bo Jiang;Kai-Yue Tang;Ying Yan;Marc F.Schetelig;Jin-Jun Wang
作者机构：
Key Laboratory of Entomology and Pest Control Engineering,College of Plant Protection,Southwest University,Chongqing,China;International Joint Laboratory of China-Belgium on Sustainable Crop Pest Control,Academy of Agricultural Sciences,Southwest Univer-sity,Chongqing,China;Department of Insect Biotechnology in Plant Protection,Justus-Liebig-University Giessen,Institute for Insect Biotechnology,Giessen,Germany
文献出处：
引用格式：
[1]Li Xu;Hong-Bo Jiang;Kai-Yue Tang;Ying Yan;Marc F.Schetelig;Jin-Jun Wang-.CRISPR-mediated mutagenesis of the odorant receptor co-receptor(Orco)gene disrupts olfaction-mediated behaviors in Bactrocera dorsalis)[J].中国昆虫科学（英文版）,2022(05):1275-1286
A类：
Olfaction,oblig,atory,BdorOrco,Electroantennograms,Oviposition,benzothiazole
B类：
CRISPR,mediated,mutagenesis,odorant,receptor,disrupts,olfaction,behaviors,Bactrocera,dorsalis,plays,essential,role,insect,such,host,location,foraging,mating,oviposition,indispensable,perception,Here,characterized,from,oriental,fruit,fly,Hendel,notorious,agricul,ture,pest,olfactory,deficiency,mutants,were,generated,by,editing,using,Cas9,system,EAG,preference,confirmed,that,flies,had,reduced,methyl,eugenol,caryophyllene,acetate,bioassays,showed,eggs,laid,females,octen,significantly,creased,In,addition,took,longer,locate,food,source,compared,wild,type,WT,Altogether,data,indicated,multiple,physiological,processes,expands,understanding,function
AB值：
0.512748
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机标中图分类号，由域田数据科技根据网络公开资料自动分析生成，仅供学习研究参考。
