Aphids are important agricultural pests,vectors of many plant viruses and have sophisticated relationships with symbiotic microorganisms.Abundant asymptomatic RNA viruses have been reported in aphids due to the application of RNA-seq,but aphid-virus interactions remain unclear.Bunyavirales is the most abundant RNA virus order,which can infect mammals,arthropods,and plants.However,many bunyaviruses have specific hosts,such as insects.Here,we discovered 18 viruses from 10 aphid species by RNA-seq.Importantly,a widespread presence bunyavirus,Aphid bunyavirus 1(ABV-1),was determined to have a wide host range,infecting and replicating in all 10 tested aphid species.ABV-1 may be transmitted horizontally during feeding on plant leaves and verti-cally through reproduction.In a comparison of the physiological parameters of ABV-lhigh and ABV-1low strains of pea aphid,higher ABV-1 titers reduced the total nymphal du-ration and induced the reproduction.Moreover,viral titer significantly affected the lipid and protein contents in pea aphids.In summary,we proposed that ABV-1 may have sta-ble symbiont-like relationships with aphids,and these observations may provide a new direction for studying bunyaviruses in aphids and establishing a model for virus-aphid interactions.

Xin An;Wei Zhang;Chao Ye;Guy Smagghe;Jin-Jun Wang;Jinzhi Niu

Key Laboratory of Entomology and Pest Control Engineering,College of Plant Protection,Southwest University,Chongqing,China;International Joint Laboratory of China-Belgium on Sustainable Crop Pest Control,Academy of Agricultural Sciences,Southwest Uni-versity,Chongqing,China;Department of Plants and Crops,Faculty of Bioscience Engineering,Ghent University,Ghent,Belgium

中国昆虫科学（英文版）

[1]Xin An;Wei Zhang;Chao Ye;Guy Smagghe;Jin-Jun Wang;Jinzhi Niu-.Discovery of a widespread presence bunyavirus that may have symbiont-like relationships with different species of aphids)[J].中国昆虫科学（英文版）,2022(04):1120-1134

symbiont,Aphids,bunyaviruses,Aphid,ABV,lhigh,1low

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