典型文献
Characterization of carotenoid biosynthetic pathway genes in the pea aphid(Acyrthosiphon pisum)revealed by heterologous complementation and RNA interference assays
文献摘要：
Carotenoids are involved in many essential physiological functions and are pro-duced from geranylgeranyl pyrophosphate through synthase,desaturase,and cyclase activ-ities.In the pea aphid(Acyrthosiphon pisum),the duplication of carotenoid biosynthetic genes,including carotenoid synthases/cyclases(ApCscA-C)and desaturases(ApCdeA-D),through horizontal gene transfer from fungi has been detected,and ApCdeB has known dehydrogenation functions.However,whether other genes contribute to aphid carotenoid biosynthesis,and its specific regulatory pathway,remains unclear.In the current study,functional analyses of seven genes were performed using heterologous complementation and RNA interference assays.The bifunctional enzymes ApCscA-C were responsible for the synthase of phytoene,and ApCscC may also have a cyclase activity.ApCdeA,ApCdeC,and ApCdeD had diverse dehydrogenation functions.ApCdeA catalyzed the en-zymatic conversion of phytoene to neurosporene(three-step product),ApCdeC catalyzed the enzymatic conversion of phytoene toξ-carotene(two-step product),and ApCdeD cat-alyzed the enzymatic conversion of phytoene to lycopene(four-step product).Silencing of ApCscs reduced the expression levels of ApCdes,and silencing these carotenoid biosyn-thetic genes reduced the α-,β-,and y-carotene levels,as well as the total carotenoid level.The results suggest that these genes were activated and led to carotenoid biosynthesis in the pea aphid.
文献关键词：
中图分类号：
作者姓名：
Bi-Yue Ding;Xiu-Cheng Xie;Feng Shang;Guy Smagghe;Jin-Zhi Niu;Jin-Jun Wang
作者机构：
Key Laboratory of Entomology and Pest Control Engineering,College of Plant Protection,Southwest University,Chongqing,China;International Joint Laboratory of China-Belgium on Sustainable Crop Pest,Control,Academy of Agricultural Sciences,Southwest Uni-versity,Chongqing,China;Department of Plants and Crops,Faculty of Bioscience Engineering,Ghent University,Ghent,Belgium
文献出处：
引用格式：
[1]Bi-Yue Ding;Xiu-Cheng Xie;Feng Shang;Guy Smagghe;Jin-Zhi Niu;Jin-Jun Wang-.Characterization of carotenoid biosynthetic pathway genes in the pea aphid(Acyrthosiphon pisum)revealed by heterologous complementation and RNA interference assays)[J].中国昆虫科学（英文版）,2022(03):645-656
A类：
ApCscA,desaturases,ApCdeA,ApCdeB,ApCscC,ApCdeC,ApCdeD,zymatic,neurosporene,ApCscs,ApCdes
B类：
Characterization,carotenoid,biosynthetic,pathway,genes,pea,aphid,Acyrthosiphon,pisum,revealed,by,heterologous,complementation,interference,assays,Carotenoids,are,involved,many,essential,physiological,functions,from,geranylgeranyl,pyrophosphate,through,ities,In,duplication,including,synthases,cyclases,horizontal,transfer,fungi,been,detected,known,dehydrogenation,However,whether,other,contribute,biosynthesis,its,specific,regulatory,remains,unclear,current,study,analyses,seven,were,performed,using,bifunctional,enzymes,responsible,phytoene,may,also,have,activity,had,diverse,catalyzed,conversion,three,step,product,enzymatic,carotene,two,lycopene,four,Silencing,reduced,expression,levels,silencing,these,well,total,results,suggest,that,activated
AB值：
0.408699
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