典型文献
A venom protein of ectoparasitoid Pachycrepoideus vindemiae,PvG6PDH,contributes to parasitism by inhibiting host glucose-6-phosphate metabolism
文献摘要：
To achieve successful development,female parasitoids,while laying eggs,in-troduce various virulence factors,mainly venoms,into host insects to manipulate their physiology.Although numerous studies have been conducted to characterize the compo-nents of venoms that regulate host immune responses,few systematic investigations have been conducted on the roles of venom proteins in host metabolic regulation.In this in-vestigation,we characterized a novel venom protein in Pachycrepoideus vindemiae called glucose-6-phosphate dehydrogenase(PvG6PDH)and showed it has a vital role in regulat-ing host carbohydrate metabolism.PvG6PDH encodes 510 amino acids and features a sig-nal peptide and two conserved"G6PDH"domains.Multiple sequence alignment showed it has high amino acid identity with G6PDH from other pteromalids,and quantitative poly-merase chain reaction analysis and immunofluorescent staining demonstrated a signifi-cantly higher expression of PvG6PDH in the venom apparatus compared with the carcass.We report that PvG6PDH contributes to parasitism by inhibiting the glucose-6-phosphate(G6P)metabolism of host Drosophila melanogaster,as demonstrated by PvG6PDH injec-tion and RNA interference analysis.Further tests revealed that the accumulation of host G6P was caused by the transcriptional inhibition of G6P-metabolism-related genes.These findings greatly contribute to our understanding of venom-mediated host metabolic regu-lation,further laying the foundation for the development of venom proteins as biological agents for pest control.
文献关键词：
中图分类号：
作者姓名：
Lei Yang;Liming Qiu;Qi Fang;Shaoying Wu;Gongyin Ye
作者机构：
Hainan University,Haikou,570228,China;State Key Laboratory of Rice Biology&Ministry of Agricultural and Rural Affairs Key Laboratory of Molecular Biology of Crop Pathogens and Insects,Institute of Insect Sciences,Zhejiang University,Hangzhou,310058,China
文献出处：
引用格式：
[1]Lei Yang;Liming Qiu;Qi Fang;Shaoying Wu;Gongyin Ye-.A venom protein of ectoparasitoid Pachycrepoideus vindemiae,PvG6PDH,contributes to parasitism by inhibiting host glucose-6-phosphate metabolism)[J].中国昆虫科学（英文版）,2022(02):399-410
A类：
ectoparasitoid,Pachycrepoideus,vindemiae,PvG6PDH,parasitoids,venoms,vestigation,pteromalids
B类：
contributes,parasitism,by,inhibiting,host,glucose,phosphate,metabolism,To,achieve,successful,development,female,while,laying,eggs,troduce,various,virulence,factors,mainly,into,insects,manipulate,their,physiology,Although,numerous,studies,have,been,conducted,compo,nents,that,regulate,immune,responses,few,systematic,investigations,roles,proteins,metabolic,regulation,In,this,characterized,novel,called,dehydrogenase,showed,has,vital,carbohydrate,encodes,amino,acids,features,peptide,two,conserved,domains,Multiple,sequence,alignment,identity,from,other,quantitative,poly,merase,chain,reaction,analysis,immunofluorescent,staining,demonstrated,signifi,cantly,higher,expression,apparatus,compared,carcass,We,report,Drosophila,melanogaster,injec,interference,Further,tests,revealed,accumulation,was,caused,transcriptional,inhibition,related,genes,These,findings,greatly,our,understanding,mediated,further,foundation,biological,agents,pest,control
AB值：
0.487885
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