The zona pellucida domain protein Dusky(Dy)plays a vital role in wing mor-phogenesis in insects,but little information on its function has been reported.In this study,we found that dy regulated wing cell size,larval and pupal duration,and the metabolism of amino acid and 20-hydroxyecdysone in Tribolium castaneum.Using RNA-seq,413 dif-ferentially expressed genes were identified between physiological buffer-injected and dy-double-stranded RNA-treated larvae,including 88 downregulated genes and 325 upregu-lated genes.Among these genes,dy knockdown increased CYP18A1 expression to elevate the 26-hydroxylation of 20-hydroxyecdysone,which ultimately led to growth defects in wing cells.Silencing of dy upregulated the transcription of genes encoding tyrosine amino-transferase,4-hydroxyphenylpyruvate dioxygenase,homogentisate 1,2-dioxygenase,and Pale to promote the catabolism of tyrosine and phenylalanine,which eventually reduced amino acid content.Furthermore,dy knockdown upregulated 4E-BP expression,and 4E-BP silencing partially phenocopied dy RNA interference-mediated wing morphogenesis.These results suggest that Dy controls 20-hydroxyecdysone and amino acid metabolism to regulate wing morphogenesis in the insect.

Chengjun Li;Liu Yang;Youwei Wang;Huanyu Du;Jiangyan Zhang;Yaoyao Lu;Bin Li;Keping Chen

School of Life Sciences,Jiangsu University,Zhenjiang,Jiangsu Province,212013,China;Jiangsu Key Laboratory for Biodiversity and Biotechnology,College of Life Sciences,Nanjing Normal University,Nanjing,210023,China

中国昆虫科学（英文版）

[1]Chengjun Li;Liu Yang;Youwei Wang;Huanyu Du;Jiangyan Zhang;Yaoyao Lu;Bin Li;Keping Chen-.Functional analysis of zona pellucida domain protein Dusky in Tribolium castaneum)[J].中国昆虫科学（英文版）,2022(02):388-398

Dusky,phogenesis,CYP18A1,hydroxyphenylpyruvate,homogentisate,Pale,phenocopied

Functional,analysis,zona,pellucida,domain,protein,Tribolium,castaneum,Dy,plays,vital,role,wing,insects,but,little,information,its,function,has,been,reported,In,this,study,found,that,size,larval,pupal,duration,metabolism,amino,acid,hydroxyecdysone,Using,seq,dif,ferentially,expressed,were,identified,between,physiological,buffer,injected,double,stranded,treated,larvae,including,downregulated,Among,these,knockdown,increased,expression,elevate,hydroxylation,which,ultimately,led,growth,defects,cells,Silencing,upregulated,transcription,encoding,tyrosine,transferase,dioxygenase,promote,catabolism,phenylalanine,eventually,reduced,content,Furthermore,4E,silencing,partially,interference,mediated,morphogenesis,These,results,suggest,controls

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