典型文献
Translation landscape of SARS-CoV-2 noncanonical subgenomic RNAs
文献摘要：
The ongoing COVID-19 pandemic is caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)with a positive-stranded RNA genome.Current proteomic studies of SARS-CoV-2 mainly focus on the proteins encoded by its genomic RNA(gRNA)or canonical subgenomic RNAs(sgRNAs).Here,we systematically inves-tigated the translation landscape of SARS-CoV-2,especially its noncanonical sgRNAs.We first constructed a strict pipeline,named vipep,for identifying reliable peptides derived from RNA viruses using RNA-seq and mass spectrometry data.We applied vipep to analyze 24 sets of mass spectrometry data related to SARS-CoV-2 infection.In addition to known canonical proteins,we identified many noncanonical sgRNA-derived peptides,which stably increase after viral infection.Furthermore,we explored the potential functions of those proteins encoded by noncanonical sgRNAs and found that they can bind to viral RNAs and may have immunogenic ac-tivity.The generalized vipep pipeline is applicable to any RNA viruses and these results have expanded the SARS-CoV-2 translation map,providing new insights for understanding the functions of SARS-CoV-2 sgRNAs.
文献关键词：
中图分类号：
作者姓名：
Kai Wu;Dehe Wang;Junhao Wang;Yu Zhou
作者机构：
State Key Laboratory of Virology,College of Life Sciences,Wuhan University,Wuhan,430072,China;TaiKang Center for Life and Medical Sciences,RNA Institute,Wuhan University,Wuhan,430072,China;Institute for Advanced Studies,Wuhan University,Wuhan,430072,China;Frontier Science Center for Immunology and Metabolism,Wuhan University,Wuhan,430072,China
文献出处：
引用格式：
[1]Kai Wu;Dehe Wang;Junhao Wang;Yu Zhou-.Translation landscape of SARS-CoV-2 noncanonical subgenomic RNAs)[J].中国病毒学,2022(06):813-822
A类：
subgenomic,vipep
B类：
Translation,landscape,SARS,CoV,noncanonical,ongoing,pandemic,is,caused,by,severe,acute,respiratory,syndrome,coronavirus,positive,stranded,genome,Current,proteomic,studies,mainly,focus,proteins,encoded,its,sgRNAs,Here,we,systematically,inves,tigated,translation,especially,We,first,constructed,strict,pipeline,named,identifying,reliable,peptides,derived,from,viruses,using,seq,mass,spectrometry,data,applied,analyze,sets,related,infection,In,addition,known,identified,many,which,stably,increase,after,viral,Furthermore,explored,potential,functions,those,found,that,they,bind,may,have,immunogenic,tivity,generalized,applicable,these,results,expanded,map,providing,new,insights,understanding
AB值：
0.521543
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