典型文献
CRISPR/Cas9-mediated genome editing reveals 12 testis-enriched genes dispensable for male fertility in mice
文献摘要：
Gene expression analyses suggest that more than 1000-2000 genes are expressed predominantly in mouse and human testes.Although functional analyses of hundreds of these genes have been performed,there are still many testis-enriched genes whose functions remain unexplored.Analyzing gene function using knockout(KO)mice is a powerful tool to discern if the gene of interest is essential for sperm formation,function,and male fertility in vivo.In this study,we generated KO mice for 12 testis-enriched genes,1700057G04Rik,4921539E11Rik,4930558C23Rik,Cby2,Ldhal6b,Rasef,Slc25a2,Slc25a41,Smim8,Smim9,Tmem210,and Tomm20I,using the clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9(CRISPR/Cas9)system.We designed two gRNAs for each gene to excise almost all the protein-coding regions to ensure that the deletions in these genes result in a null mutation.Mating tests of KO mice reveal that these 12 genes are not essential for male fertility,at least when individually ablated,and not together with other potentially compensatory paralogous genes.Our results could prevent other laboratories from expending duplicative effort generating KO mice,for which no apparent phenotype exists.
文献关键词：
中图分类号：
作者姓名：
Yuki Oyama;Haruhiko Miyata;Keisuke Shimada;Yoshitaka Fujihara;Keizo Tokuhiro;Thomas X Garcia;Martin M Matzuk;Masahito Ikawa
作者机构：
Graduate School of Pharmaceutical Sciences,Osaka University,Suita,Osaka 565-0871,Japan;Department of Experimental Genome Research,Research Institute for Microbial Diseases,Osaka University,Suita,Osaka 565-0871,Japan;Department of Bioscience and Genetics,National Cerebral and Cardiovascular Center,Suita,Osaka 564-8565,Japan;Department of Genome Editing,Institute of Biomedical Science,Kansai Medical University,Hirakata,Osaka 573-1191,Japan;Center for Drug Discovery,Baylor College of Medicine,Houston,TX 77030,USA;Department of Pathology and Immunology,Baylor College of Medicine,Houston,TX 77030,USA;Department of Biology and Biotechnology,University of Houston-Clear Lake,Houston,TX 77058,USA;The Institute of Medical Science,The University of Tokyo,Minato-ku,Tokyo 108-8639,Japan
文献出处：
引用格式：
[1]Yuki Oyama;Haruhiko Miyata;Keisuke Shimada;Yoshitaka Fujihara;Keizo Tokuhiro;Thomas X Garcia;Martin M Matzuk;Masahito Ikawa-.CRISPR/Cas9-mediated genome editing reveals 12 testis-enriched genes dispensable for male fertility in mice)[J].亚洲男性学杂志（英文版）,2022(03):266-272
A类：
1700057G04Rik,4921539E11Rik,4930558C23Rik,Cby2,Ldhal6b,Rasef,Slc25a2,Slc25a41,Smim8,Smim9,Tmem210,Tomm20I,gRNAs,excise,paralogous,expending,duplicative
B类：
CRISPR,Cas9,mediated,genome,editing,reveals,testis,enriched,genes,dispensable,male,fertility,mice,Gene,expression,analyses,suggest,that,more,than,expressed,predominantly,mouse,human,testes,Although,functional,hundreds,these,have,been,performed,there,still,many,whose,functions,remain,unexplored,Analyzing,using,knockout,KO,powerful,tool,discern,if,interest,essential,sperm,formation,vivo,In,this,study,generated,clustered,regularly,interspaced,short,palindromic,repeats,associated,protein,system,We,designed,two,each,almost,coding,regions,ensure,deletions,null,mutation,Mating,tests,least,when,individually,ablated,together,other,potentially,compensatory,Our,results,could,prevent,laboratories,from,effort,generating,which,apparent,phenotype,exists
AB值：
0.512312
相似文献
机标中图分类号，由域田数据科技根据网络公开资料自动分析生成，仅供学习研究参考。
