Plant receptor-like kinases(RLKs)regulate many processes in plants.Many RLKs perform significant roles in plant immunity.Lectin receptor-like kinases(LecRLKs)are a large family of RLKs.However,the function of most LecRLKs is poorly understood.In this study,we show that a potato LecRLK,StLecRK-Ⅳ.1,is involved in plant immunity against Phytophthora infestans.As a negative regulator of immunity,StLecRK-Ⅳ.1 is downregulated by P.infestans and activated by abscisic acid.The transient expression of StLecRK-Ⅳ.1 in Nicotiana benthamiana enhanced P.infestans leaf colonization significantly.In contrast,the size of disease lesions caused by P.infestans was reduced by virus-induced gene silencing of the StLecRK-Ⅳ.1 ortholog in N.benthamiana,NbLecRK-Ⅳ.1,as well as in potato plants with stable RNA interference of StLecRK-Ⅳ.1.Tetraspanin-8(StTET8)was identified to be interacting with StLecRK-Ⅳ.1 using a membrane yeast two-hybrid system,which was further verified by co-immunoprecipitation,a luciferase complementation assay,and a bimolecular fluorescence complementation test.StTET8 is a positive immune regulator that restrains P.infestans infection.The co-expression of StLecRK-Ⅳ.1 with StTET8 antagonized the positive roles of StTET8 against P.infestans.Moreover,the co-expression of StTET8 with StLecRK-Ⅳ.1 affected the stability of StTET8,which was confirmed by a western blot assay and confocal assay.Taken together,our work first reveal that a potato L-type lectin RLK,StLecRK-Ⅳ.1,negatively regulates plant immunity by targeting a positive regulator,StTET8,through affecting its stability.

Lei Guo;Yetong Qi;Yang Mu;Jing Zhou;Wenhe Lu;Zhendong Tian

College of Agronomy,Northeast Agricultural University,Harbin,150030,China;Key Laboratory of Horticultural Plant Biology(HZAU),Ministry of Education,Huazhong Agricultural University(HZAU),Wuhan,430070,China;Key Laboratory of Potato Biology and Biotechnology(HZAU),Ministry of Agriculture and Rural Affairs,Huazhong Agricultural University,Wuhan,430070,China;Potato Engineering and Technology Research Center of Hubei Province,Huazhong Agricultural University,Wuhan,430070,China;Hubei Hongshan Laboratory.Huazhong Agricultural University,Wuhan,430070,China

园艺研究（英文）

[1]Lei Guo;Yetong Qi;Yang Mu;Jing Zhou;Wenhe Lu;Zhendong Tian-.Potato StLecRK-Ⅳ.1 negatively regulates late blight resistance by affecting the stability of a positive regulator StTET8)[J].园艺研究（英文）,2022(05):82-95

StLecRK,StTET8,LecRLKs,LecRLK,NbLecRK,Tetraspanin

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