典型文献
Characterization and functional validation of β-carotene hydroxylase AcBCH genes in Actinidia chinensis
文献摘要：
Carotenoids are the pigment substances of yellow-fleshed kiwifruit,and among them β-cryptoxanthin has only been detected in the brighter yellow-fleshed variety'Jinshi 1'.β-Carotene hydroxylase(BCH)catalyzes the formation of β-cryptoxanthin and zeaxanthin,but its molecular characteristics and functions have not been fully explained.Here we isolated two β-carotene hydroxylase genes,AcBCH1 and AcBCH2 from kiwifruit(Actinidia chinensis),and their relative expression levels exhibited a close correlation with the content of β-cryptoxanthin.AcBCH1 catalyzed the formation of β-cryptoxanthin when transformed into β-carotene-accumulating yeast cells.Moreover,silenced expression of AcBCH1 in kiwifruit caused decreases in the contents of zeaxanthin,lutein,andβ-cryptoxanthin,and an increase in β-carotene content.The content of β-carotene decreased significantly after the AcBCH1/2 genes were overexpressed in tomato.The content of zeaxanthin increased and β-carotene decreased in transgenic kiwifruit seedlings.The results will enrich our knowledge of the molecular mechanisms of carotenoid biosynthesis in kiwifruit.
文献关键词：
中图分类号：
作者姓名：
Hui Xia;Yuanjie Zhou;Zhiyi Lin;Yuqi Guo;Xinling Liu;Tong Wang;Jin Wang;Honghong Deng;Lijin Lin;Qunxian Deng;Xiulan Lv;Kunfu Xu;Dong Liang
作者机构：
College of Horticulture,Sichuan Agricultural University,Chengdu 611130,China;Institute of Pomology and Olericulture,Sichuan Agricultural University,Chengdu 611130,China
文献出处：
引用格式：
[1]Hui Xia;Yuanjie Zhou;Zhiyi Lin;Yuqi Guo;Xinling Liu;Tong Wang;Jin Wang;Honghong Deng;Lijin Lin;Qunxian Deng;Xiulan Lv;Kunfu Xu;Dong Liang-.Characterization and functional validation of β-carotene hydroxylase AcBCH genes in Actinidia chinensis)[J].园艺研究（英文）,2022(01):455-464
A类：
AcBCH,fleshed,cryptoxanthin,Jinshi,AcBCH1,AcBCH2
B类：
Characterization,functional,validation,carotene,hydroxylase,genes,Actinidia,chinensis,Carotenoids,are,pigment,substances,yellow,kiwifruit,among,them,has,only,been,detected,brighter,variety,Carotene,catalyzes,formation,zeaxanthin,but,its,molecular,characteristics,functions,have,not,fully,explained,Here,isolated,two,from,their,relative,expression,levels,exhibited,close,correlation,catalyzed,when,transformed,into,accumulating,yeast,cells,Moreover,silenced,caused,decreases,contents,lutein,decreased,significantly,after,were,overexpressed,tomato,increased,transgenic,seedlings,results,will,enrich,our,knowledge,mechanisms,carotenoid,biosynthesis
AB值：
0.49267
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