典型文献
Creation of artificial karyotypes in mice reveals robustness of genome organization
文献摘要：
Dear Editor,
Robertsonian(Rb)fusions contribute to the diversity in species karyotypes.However,how it occurs and propels evolution is still unknown,mainly due to the lack of a simple and efficient method for genetically manipulating chromosomes in animals.While the typical mouse karyotype(2n=40)is completely telocentric except chromo-some Y(ChrY),the western European subspecies M.m.domesticus contains diverse chromosomal races,with a diploid number varying between 38 and 22 due to carrying one or multiple pairs of metacentric chromosomes,which results from the Rb fusions between two telocentric chromosomes.1 Recapitulation of this process in the laboratory may help to shed light on the function of Rb fusions in evolution.The detailed mechanisms underlying Rb fusion in M.m.domesticus requires further investigations,2 but previous studies have shown that Rb fusion-induced metacentric chromosomes have a neo-centromeric region of minor satellite(MinSat)sandwiched between two large blocks of major satellite(MajSat)DNA.3 These observations imply that Rb fusion breakpoints are localized within the MinSat sequences of the centromeric region.Given that CRISPR-Cas9-mediated chromosome engineering has successfully fused 16 yeast chromosomes through head-to-tail strategies to produce yeast strains with 1 or 2 chromosomes,4,5 we hypothesize that CRISPR-Cas9-mediated chromosome engineering through targeting MinSat sequences could be an ideal strategy for creating mouse strains with reduced chromosome numbers in the laboratory.
文献关键词：
中图分类号：
作者姓名：
Xiaoyu Merlin Zhang;Meng Yan;Zhenhua Yang;Hao Xiang;Wei Tang;Xindong Cai;Qigui Wu;Xin Liu;Gang Pei;Jinsong Li
作者机构：
State Key Laboratory of Cell Biology,Shanghai Key Laboratory of Molecular Andrology,Shanghai Institute of Biochemistry and Cell Biology,CAS Center for Excellence in Molecular Cell Science,University of Chinese Academy of Sciences,Chinese Academy of Sciences,Shanghai,China;School of Life Science,Hangzhou Institute for Advanced Study,University of Chinese Academy of Sciences,Hangzhou,Zhejiang,China;School of Life Science and Technology,ShanghaiTech University,Shanghai,China;State Key Laboratory of Molecular Biology,Shanghai Institute of Biochemistry and Cell Biology,CAS Center for Excellence in Molecular Cell Science,University of Chinese Academy of Sciences,Chinese Academy of Sciences,Shanghai,China
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引用格式：
[1]Xiaoyu Merlin Zhang;Meng Yan;Zhenhua Yang;Hao Xiang;Wei Tang;Xindong Cai;Qigui Wu;Xin Liu;Gang Pei;Jinsong Li-.Creation of artificial karyotypes in mice reveals robustness of genome organization)[J].细胞研究（英文版）,2022(11):1026-1029
A类：
karyotypes,
Robertsonian,propels,telocentric,ChrY,metacentric,Recapitulation,centromeric,MinSat,MajSat
B类：
Creation,artificial,mice,reveals,robustness,genome,organization,Dear,Editor,Rb,fusions,contribute,diversity,However,occurs,evolution,still,unknown,mainly,due,lack,simple,efficient,method,genetically,manipulating,chromosomes,animals,While,typical,mouse,2n,completely,except,western,European,subspecies,domesticus,contains,diverse,chromosomal,races,diploid,varying,between,carrying,one,multiple,pairs,which,results,from,two,this,process,laboratory,may,help,shed,light,function,detailed,mechanisms,underlying,requires,further,investigations,previous,studies,have,shown,that,induced,neo,region,minor,satellite,sandwiched,large,blocks,major,These,observations,imply,breakpoints,are,localized,within,sequences,Given,CRISPR,Cas9,mediated,engineering,has,successfully,fused,yeast,through,head,strategies,produce,strains,hypothesize,targeting,could,ideal,strategy,creating,reduced,numbers
AB值：
0.509435
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