典型文献
Temporal profiling with ultra-deep RRBS sequencing reveals the relative rarity of stably maintained methylated CpG sites in human cells
文献摘要：
Methylation of cytosine within cytosine-guanine(CpG)sites is one of the most common forms of epigenetic regulation of genomic processes in mammals[1].As such,faithful transmission of CpG methylation following DNA replication is believed to be essential for the maintenance of cell phenotype and function[2].However,it is also known that DNA methylation is a dynamic process at a number of CpG sites during cell passaging.In fact,partial methylation is quite common at the population level,implying that there is considerable heterogeneity in methylation at many CpG sites among individual cells in the population.Thus,it is possible that only a portion of all CpG sites must remain methylated in all of the cells in the population to maintain the phenotypic identity and functional state of each cell.However,to date,it remains largely unknown what proportion of CpG sites in the genome are stably maintained in methylation during cell passaging owing to the relatively low depth of sequencing of most datasets and the absence of rigorous,well-controlled experimental conditions to examine this specific issue.In this study,we sought to identify CpG sites whose methylation is stably maintained during cell passaging on a global scale and explore their potential implications and possible clinical applica-tions.
文献关键词：
中图分类号：
作者姓名：
Jianing Wang;Yulan Qin;Yani Kang;Xinhui Li;Yuan Wang;Hua Li;Daniel M.Czajkowsky;Zhifeng Shao
作者机构：
State Key Laboratory for Oncogenes and Bio-ID Center,School of Biomedical Engineering,Shanghai Jiao Tong University,Shanghai 200240,China
文献出处：
引用格式：
[1]Jianing Wang;Yulan Qin;Yani Kang;Xinhui Li;Yuan Wang;Hua Li;Daniel M.Czajkowsky;Zhifeng Shao-.Temporal profiling with ultra-deep RRBS sequencing reveals the relative rarity of stably maintained methylated CpG sites in human cells)[J].生物化学与生物物理学报（英文版）,2022(12):1935-1938
A类：
passaging
B类：
Temporal,profiling,ultra,deep,RRBS,sequencing,reveals,rarity,stably,maintained,methylated,CpG,sites,human,cells,Methylation,cytosine,within,guanine,one,most,common,forms,epigenetic,regulation,genomic,processes,mammals,such,faithful,transmission,methylation,following,replication,believed,essential,maintenance,phenotype,However,also,that,dynamic,number,during,In,fact,partial,quite,population,level,implying,there,considerable,heterogeneity,many,among,individual,Thus,possible,only,all,must,phenotypic,identity,functional,state,each,date,remains,largely,unknown,what,proportion,genome,are,relatively,depth,datasets,absence,rigorous,well,controlled,experimental,conditions,examine,this,specific,issue,study,sought,identify,whose,global,scale,explore,their,potential,implications,clinical,applica
AB值：
0.518769
相似文献
机标中图分类号，由域田数据科技根据网络公开资料自动分析生成，仅供学习研究参考。
