典型文献
Efficient TurboID-based proximity labelling method for identifying terminal sialic acid glycosylation in living cells
文献摘要：
TurboID,a proximity labelling method based on mutant biotin ligase,is an efficient new technique for recognizing protein-protein interactions and has been successfully applied to living cells.Sialic acid is typically the terminal monosaccharide attached to many glycoproteins and plays many important roles in many biological processes.However,the lack of enrichment methods for terminal sialic acid glycosylation in vivo hinders the identification and analysis of this glycosylation.Here,we introduce TurboID to identify terminal sialic acid glycosylation in living cells.SpCBM,the carbohydrate-binding domain of sialidase from Streptococcus pneumoniae,is fused with TurbolD and overexpressed in HeLa cells.After streptavidin-based purification and detection by mass spectrometry,31 terminal sialic acid N-glycosylated sites and 1359 putative terminal sialic acid glycosylated proteins are identified,many of which are located in the cytoplasm and nucleus.
文献关键词：
中图分类号：
作者姓名：
Wu Liu;Yunfeng Long;Yongfen Bao;Yang Li;Mengxue Deng;Xiuyi Yang;He Zhu;Yanting Su
作者机构：
Hubei Key Laboratory of Diabetes and Angiopathy,Xianning Medical Colloge,Hubei University of Science and Technology,Xianning 437100,China;School of Pharmacy,Xianning Medical Colloge,Hubei University of Science and Technology,Xianning 437100,China;School of Basic Medical Sciences,Xianning Medical Colloge,Hubei University of Science and Technology,Xianning 437100,China;Colllege of Life Sciences,Wuhan University,Wuhan 430072,China;Hepatic Surgery Center,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China;Hubei Key Laboratory of Hepato-Pancreato-Biliary Diseases,Wuhan 430030,China
文献出处：
引用格式：
[1]Wu Liu;Yunfeng Long;Yongfen Bao;Yang Li;Mengxue Deng;Xiuyi Yang;He Zhu;Yanting Su-.Efficient TurboID-based proximity labelling method for identifying terminal sialic acid glycosylation in living cells)[J].生物化学与生物物理学报（英文版）,2022(12):1841-1853
A类：
SpCBM,sialidase,TurbolD
B类：
Efficient,TurboID,proximity,labelling,identifying,terminal,sialic,acid,glycosylation,living,cells,mutant,biotin,ligase,efficient,new,technique,recognizing,interactions,has,been,successfully,applied,Sialic,typically,monosaccharide,attached,many,glycoproteins,plays,important,roles,biological,processes,However,lack,enrichment,methods,vivo,hinders,identification,analysis,this,Here,introduce,carbohydrate,binding,domain,from,Streptococcus,pneumoniae,fused,overexpressed,HeLa,After,streptavidin,purification,detection,by,mass,spectrometry,glycosylated,sites,putative,are,identified,which,located,cytoplasm,nucleus
AB值：
0.540202
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机标中图分类号，由域田数据科技根据网络公开资料自动分析生成，仅供学习研究参考。
