典型文献
A thermophilic 8-oxoguanine DNA glycosylase from Thermococcus barophiluss Ch5 is a new member of AGOG DNA glycosylase family
文献摘要：
8-Oxoguanine(8oxoG)in DNA is a major oxidized base that poses a severe threat to genome stability.To counteract the mutagenic effect generated by 8oxoG in DNA,cells have evolved 8oxoG DNA glycosylase(OGG)that can excise this oxidized base from DNA.Currently,OGG enzymes have been divided into three families:OGG1,OGG2 and AGOG(archaeal 8oxoG DNA glycosylase).Due to the limited reports,our understanding on AGOG enzymes remains incomplete.Herein,we present evidence that an AGOG from the hyperthermophilic euryarchaeon Thermo Coccus barophilus Ch5(Tb-AGOG)excises 8oxoG from DNA at high temperature.The enzyme displays maximum efficiency at 75℃-95℃and at pH 9.0.As expected,Tb-AGOG is a bifunctional glycosylase that harbors glycosylase activity and AP(apurinic/apyrimidinic)lyase activity.Importantly,we reveal for the first time that residue D41 in Tb-AGOG is essential for 8oxoG excision and intermediate formation,but not essential for DNA binding or AP cleavage.Fur-thermore,residue E79 in Tb-AGOG is essential for 8oxoG excision and intermediate formation,and is partially involved in DNA binding and AP cleavage,which has not been described among the reported AGOG members to date.Overall,our work provides new insights into catalytic mechanism of AGOG enzymes.
文献关键词：
中图分类号：
作者姓名：
Lei Wang;Donghao Jiang;Likui Zhang
作者机构：
College of Environmental Science and Engineering,Marine Science&Technology Institute,Yangzhou University,Yangzhou 225127,China;Guangling College,Yangzhou University,Yangzhou 225000,China
文献出处：
引用格式：
[1]Lei Wang;Donghao Jiang;Likui Zhang-.A thermophilic 8-oxoguanine DNA glycosylase from Thermococcus barophiluss Ch5 is a new member of AGOG DNA glycosylase family)[J].生物化学与生物物理学报（英文版）,2022(12):1801-1810
A类：
oxoguanine,barophiluss,Ch5,AGOG,Oxoguanine,8oxoG,OGG,excise,OGG2,hyperthermophilic,euryarchaeon,Coccus,barophilus,excises,apurinic,apyrimidinic,D41,E79
B类：
glycosylase,from,Thermococcus,new,family,major,oxidized,base,that,poses,severe,threat,genome,stability,To,counteract,mutagenic,effect,generated,by,cells,have,evolved,can,this,Currently,enzymes,been,divided,into,three,families,OGG1,archaeal,Due,limited,reports,our,understanding,remains,incomplete,Herein,we,present,evidence,Tb,high,temperature,displays,maximum,efficiency,expected,bifunctional,harbors,activity,AP,lyase,Importantly,reveal,first,residue,essential,excision,intermediate,formation,but,not,binding,cleavage,Fur,thermore,partially,involved,which,has,described,among,reported,members,date,Overall,work,provides,insights,catalytic,mechanism
AB值：
0.378304
相似文献
机标中图分类号，由域田数据科技根据网络公开资料自动分析生成，仅供学习研究参考。
