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典型文献
A live attenuated virus-based intranasal COVID-19 vaccine provides rapid,prolonged,and broad protection against SARS-CoV-2
文献摘要:
Remarkable progress has been made in developing intramuscular vaccines against severe acute respira-tory syndrome coronavirus 2(SARS-CoV-2);however,they are limited with respect to eliciting local immunity in the respiratory tract,which is the primary infection site for SARS-CoV-2.To overcome the limitations of intramuscular vaccines,we constructed a nasal vaccine candidate based on an influenza vector by inserting a gene encoding the receptor-binding domain(RBD)of the spike protein of SARS-CoV-2,named CA4-dNS1-nCoV-RBD(dNS1-RBD).A preclinical study showed that in hamsters challenged 1 d after single-dose vaccination or 9 months after booster vaccination,dNS1-RBD largely mitigated lung pathology,with no loss of body weight.Moreover,such cellular immunity is relatively unimpaired for the most concerning SARS-CoV-2 variants,especially for the latest Omicron variant.In addition,this vaccine also provides cross-protection against H1N1 and H5N1 influenza viruses.The protective immune mech-anism of dNS1-RBD could be attributed to the innate immune response in the nasal epithelium,local RBD-specific T cell response in the lung,and RBD-specific IgA and IgG response.Thus,this study demon-strates that the intranasally delivered dNS1-RBD vaccine candidate may offer an important addition to the fight against the ongoing coronavirus disease 2019 pandemic and influenza infection,compensating limitations of current intramuscular vaccines.
文献关键词:
作者姓名:
Junyu Chen;Pui Wang;Lunzhi Yuan;Liang Zhang;Limin Zhang;Hui Zhao;Congjie Chen;Xijing Wang;Jinle Han;Yaode Chen;Jizong Jia;Zhen Lu;Junping Hong;Zicen Lu;Qian Wang;Rirong Chen;Ruoyao Qi;Jian Ma;Min Zhou;Huan Yu;Chunlan Zhuang;Xiaohui Liu;Qiangyuan Han;Guosong Wang;Yingying Su;Quan Yuan;Tong Cheng;Ting Wu;Xiangzhong Ye;Tianying Zhang;Changgui Li;Jun Zhang;Huachen Zhu;Yixin Chen;Honglin Chen;Ningshao Xia
作者机构:
State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics,National Institute of Diagnostics and Vaccine Development in Infectious Diseases,School of Life Sciences,School of Public Health,Xiamen University,Xiamen 361102,China;State Key Laboratory of Emerging Infectious Diseases,School of Public Health,Li Ka Shing Faculty of Medicine,The University of Hong Kong,Hong Kong 999077,China;National Institute for Food and Drug Control,Beijing 102629,China;Beijing Wantai Biological Pharmacy Enterprise Co.,Ltd.,Beijing 102206,China;Guangdong-Hong Kong Joint Laboratory of Emerging Infectious Diseases/Joint Laboratory for International Collaboration in Virology and Emerging Infectious Diseases,Joint Institute of Virology(STU/HKU),Shantou University,Shantou 515063,China;EKIH Pathogen Research Institute,Shenzhen 518067,China
引用格式:
[1]Junyu Chen;Pui Wang;Lunzhi Yuan;Liang Zhang;Limin Zhang;Hui Zhao;Congjie Chen;Xijing Wang;Jinle Han;Yaode Chen;Jizong Jia;Zhen Lu;Junping Hong;Zicen Lu;Qian Wang;Rirong Chen;Ruoyao Qi;Jian Ma;Min Zhou;Huan Yu;Chunlan Zhuang;Xiaohui Liu;Qiangyuan Han;Guosong Wang;Yingying Su;Quan Yuan;Tong Cheng;Ting Wu;Xiangzhong Ye;Tianying Zhang;Changgui Li;Jun Zhang;Huachen Zhu;Yixin Chen;Honglin Chen;Ningshao Xia-.A live attenuated virus-based intranasal COVID-19 vaccine provides rapid,prolonged,and broad protection against SARS-CoV-2)[J].科学通报(英文版),2022(13):1372-1387
A类:
dNS1,unimpaired
B类:
attenuated,provides,rapid,prolonged,broad,protection,against,SARS,Remarkable,progress,has,been,made,developing,intramuscular,vaccines,severe,acute,syndrome,coronavirus,however,they,are,limited,respect,eliciting,local,immunity,respiratory,tract,which,primary,infection,site,To,overcome,limitations,constructed,candidate,influenza,vector,by,inserting,gene,encoding,receptor,binding,domain,RBD,spike,protein,named,CA4,nCoV,preclinical,study,showed,that,hamsters,challenged,after,single,dose,vaccination,months,booster,largely,mitigated,lung,pathology,no,loss,body,weight,Moreover,such,cellular,relatively,most,concerning,variants,especially,latest,Omicron,In,addition,this,also,cross,H1N1,H5N1,viruses,protective,immune,mech,anism,could,attributed,innate,response,epithelium,specific,IgA,IgG,Thus,demon,strates,intranasally,delivered,may,offer,important,fight,ongoing,disease,pandemic,compensating,current
AB值:
0.553802
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