典型文献
Toward a molecular mechanism-based prediction of CRISPR-Cas9 targeting effects
文献摘要：
The CRISPR-Cas9 system,serving as a powerful genome-editing technology,has revolutionized the life sciences.However,it exhi-bits off-target activities that may present severe problems in clin-ical applications.Although a great number of in silico models have been developed to predict CRISPR targeting efficiency and speci-ficity,they are running into a bottleneck with the lack of a mech-anistic understanding of the on-and off-target activities of Cas9.Therefore,we decipher the targeting activities of Cas9 from a molecular mechanism perspective.We expect that a combination of the features derived from the molecular mechanism of Cas9 with state-of-the-art computational modeling will facilitate the development of a better in silico prediction of CRISPR-Cas9 on-/off-target activities.
文献关键词：
中图分类号：
作者姓名：
Qinchang Chen;Guohui Chuai;Chao Zhang;Qing Zhang;Qi Liu
作者机构：
Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine,Shanghai East Hospital,Bioinformatics Department,School of Life Sciences and Technology,Tongji University,Shanghai 200092,China;Shanghai Research Institute for Intelligent Autonomous Systems,Shanghai 201210,China;Department of Plastic and Reconstructive Surgery,Shanghai Institute of Precision Medicine,Shanghai Ninth People's Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200025,China;Roche Pharma Research and Early Development,pRED Informatics,Roche Innovation Center Shanghai,Roche R&D Center(China)Ltd.,Shanghai 201203,China
文献出处：
引用格式：
[1]Qinchang Chen;Guohui Chuai;Chao Zhang;Qing Zhang;Qi Liu-.Toward a molecular mechanism-based prediction of CRISPR-Cas9 targeting effects)[J].科学通报（英文版）,2022(12):1201-1204
A类：
B类：
Toward,molecular,mechanism,prediction,CRISPR,Cas9,targeting,effects,system,serving,powerful,genome,editing,technology,has,revolutionized,life,sciences,However,exhi,bits,off,activities,that,may,present,severe,problems,clin,ical,applications,Although,great,number,silico,models,have,been,developed,efficiency,speci,ficity,they,are,running,into,bottleneck,lack,anistic,understanding,Therefore,decipher,from,perspective,We,expect,combination,features,derived,state,art,computational,modeling,will,facilitate,development,better
AB值：
0.623125
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