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典型文献
Quantum dots assisted in vivo two-photon microscopy with NIR-Ⅱ emission
文献摘要:
With the advantages of high resolution and deep penetration depth,two-photon excited NIR-Ⅱ(900-1880 nm)fluorescence(2PF)microscopic bioimaging is promising.However,due to the lack of imaging systems and suit-able probes,few such works,to our best knowledge,were demonstrated utilizing NIR-Ⅱ excitation and NIR-Ⅱ fluorescence simultaneously.Herein,we used aqueously dispersible PbS/CdS quantum dots with bright NIR-Ⅱ fluorescence as the contrast agents.Under the excitation of a 1550 nm femtosecond(fs)laser,they emitted bright 2PF in the NIR-Ⅱ region.Moreover,a 2PF lifetime imaging microscopic(2PFLIM)system was implemented,and in vivo 2PFLIM images of mouse brain blood vessels were obtained for the first time to our best knowledge.To improve imaging speed,an in vivo two-photon fluorescence microscopy(2PFM)system based on an InGaAs camera was implemented,and in vivo 2PFM images of QDs-stained mouse brain blood vessels were obtained.
文献关键词:
作者姓名:
HUWEI NI;YALUN WANG;TAO TANG;WENBIN Yu;DONGYU LI;MUBIN HE;RUNZE CHEN;MINGXI ZHANG;JUN QIAN
作者机构:
State Key Laboratory of Modem Optical Instrumentations,Centre for Optical and Electromagnetic Research,College of Optical Science and Engineering,International Research Center for Advanced Photonics,Zhejiang University,Hangzhou 310058,China;SIEE(Sussex Al Institute),Zhejiang Gongshang University,Hangzhou 310018,China;State Key Laboratory of Advanced Technology for Materials Synthesis and Processing,Wuhan University of Technology,Wuhan 430070,China;Britton Chance Center for Biomedical Photonics,Wuhan National Laboratory for Optoelectronics,Huazhong University of Science and Technology,Wuhan 430074,China
引用格式:
[1]HUWEI NI;YALUN WANG;TAO TANG;WENBIN Yu;DONGYU LI;MUBIN HE;RUNZE CHEN;MINGXI ZHANG;JUN QIAN-.Quantum dots assisted in vivo two-photon microscopy with NIR-Ⅱ emission)[J].光子学研究(英文),2022(01):189-196
A类:
2PF,aqueously,2PFLIM,2PFM
B类:
Quantum,dots,assisted,vivo,two,photon,microscopy,NIR,emission,With,advantages,high,resolution,deep,penetration,depth,excited,fluorescence,microscopic,bioimaging,promising,However,due,lack,systems,suit,able,probes,few,such,works,our,best,knowledge,were,demonstrated,utilizing,excitation,simultaneously,Herein,used,dispersible,PbS,CdS,quantum,bright,contrast,agents,Under,femtosecond,fs,laser,they,emitted,region,Moreover,lifetime,was,implemented,images,mouse,brain,blood,vessels,obtained,first,To,improve,speed,InGaAs,camera,QDs,stained
AB值:
0.512793
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