典型文献
Slide-free histological imaging by microscopy with ultraviolet surface excitation using speckle illumination
文献摘要：
Microscopy with ultraviolet surface excitation(MUSE)is a promising slide-free imaging technique to improve the time-consuming histopathology workflow.However,since the penetration depth of the excitation light is tissue dependent,the image contrast could be significantly degraded when the depth of field of the imaging system is shallower than the penetration depth.High-resolution cellular imaging normally comes with a shallow depth of field,which also restricts the tolerance of surface roughness in biological specimens.Here we propose the in-corporation of MUSE with speckle illumination(termed MUSES),which can achieve sharp imaging on thick and rough specimens.Our experimental results demonstrate the potential of MUSES in providing histological images with～1 μm spatial resolution and improved contrast,within 10 minutes for a field of view of 1.7 mm×1.2 mm.With the extended depth of field feature,MUSES also relieves the constraint of tissue flatness.Furthermore,with a color transformation assisted by deep learning,a virtually stained histological image can be generated without manual tuning,improving the applicability of MUSES in clinical settings.
文献关键词：
中图分类号：
作者姓名：
IVY H.M.WONG;YAN ZHANG;ZHENGHUI CHEN;LEI KANG;TERENCE T.W.WONG
作者机构：
Translational and Advanced Bioimaging Laboratory,Department of Chemical and Biological Engineering,Hong Kong University of Science and Technology,Hong Kong,China
文献出处：
引用格式：
[1]IVY H.M.WONG;YAN ZHANG;ZHENGHUI CHEN;LEI KANG;TERENCE T.W.WONG-.Slide-free histological imaging by microscopy with ultraviolet surface excitation using speckle illumination)[J].光子学研究（英文）,2022(01):120-125
A类：
MUSES
B类：
Slide,free,histological,imaging,by,microscopy,ultraviolet,surface,excitation,using,speckle,illumination,Microscopy,promising,slide,technique,consuming,histopathology,workflow,However,since,penetration,depth,light,tissue,dependent,contrast,could,be,significantly,degraded,when,field,system,shallower,than,High,resolution,cellular,normally,comes,which,also,restricts,tolerance,roughness,biological,specimens,Here,propose,corporation,termed,achieve,sharp,thick,Our,experimental,results,demonstrate,potential,providing,images,spatial,improved,within,minutes,view,With,extended,feature,relieves,constraint,flatness,Furthermore,color,transformation,assisted,deep,learning,virtually,stained,generated,without,manual,tuning,improving,applicability,clinical,settings
AB值：
0.573362
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