Bone defects resulting from trauma,surgery,congenital malformations,and other factors are among the most common health problems nowadays.Although current strategies such as autografts and allografts are recognized as the most successful treatments for stimulating bone regeneration,limitations such as graft source and complications still exist.SmartBone? is a xeno-hybrid bone graft(made from bovine bone matrix,poly(L-lactic-co-ε-caprolactone),and gelatin)with a positive clinical record for bone regen-eration.In this study,the formulation for designing xeno-hybrid bone grafts using gelatins from different sources(bovine-and porcine-derived gelatin,with bone grafts named SBN and SPK,respectively)was investigated,and the biological responses were evaluated in vitro and in vivo.The results demonstrate that gelatins from both bovine and porcine sources can be loaded onto SmartBone? successfully and safely,withstanding the aggressive manufacturing processes.Different bone cell responses were observed in vitro.SBN was found to enhance osteocalcin secretion while SPK was found to upregulate osteopontin from human osteoblasts.In vivo,both bone grafts promoted osteogenesis,but SPK degraded earlier than SBN.Our findings suggest that SBN and SPK provide different yet comparable solutions for optimizing the bone resorption and regeneration balance.These xeno-hybrid bone grafts possess ideal potential for bone defect repairing.

Hao Zhu;H?vard Jostein Haugen;Giuseppe Perale;Janne Elin Reseland;Liebert Parreiras Nogueira;Antonio Gonzalez Cantalapiedra;Fernando Maria Guzon Mu?oz;Maria Permuy Menda?a;Felice Betge;St?le Petter Lyngstadaas;Jun Xiao

Department of Orthopedic Surgery,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China;Department of Biomaterials,Institute of Clinical Dentistry,University of Oslo,Oslo 0317,Norway;Industrie Biomediche Insubri SA,Mezzovico-Vira 6805,Switzerland;Ludwig Boltzmann Institute for Experimental and Clinical Traumatology,Vienna 1200,Austria;Faculty of Biomedical Sciences,University of Southern Switzerland,Lugano 6900,Switzerland;Faculty of Veterinary,University of Santiago de Compostela,Lugo 27002,Spain

工程（英文）

[1]Hao Zhu;H?vard Jostein Haugen;Giuseppe Perale;Janne Elin Reseland;Liebert Parreiras Nogueira;Antonio Gonzalez Cantalapiedra;Fernando Maria Guzon Mu?oz;Maria Permuy Menda?a;Felice Betge;St?le Petter Lyngstadaas;Jun Xiao-.Tailoring Resorption Rates and Osteogenic Response in Xeno-Hybrid Bone Grafts:The Effect of Added Gelatins)[J].工程（英文）,2022(06):197-208

Resorption,Grafts,Gelatins,SmartBone,gelatins

Tailoring,Rates,Osteogenic,Response,Xeno,Hybrid,Effect,Added,defects,resulting,from,trauma,surgery,congenital,malformations,other,factors,are,among,most,common,health,problems,nowadays,Although,current,strategies,such,autografts,allografts,recognized,treatments,stimulating,bone,regeneration,limitations,complications,still,exist,xeno,hybrid,made,bovine,matrix,poly,lactic,caprolactone,positive,clinical,record,In,this,study,formulation,designing,using,different,sources,porcine,derived,named,SBN,SPK,respectively,was,investigated,biological,responses,were,evaluated,vitro,vivo,results,demonstrate,that,both,can,be,loaded,onto,successfully,safely,withstanding,aggressive,manufacturing,processes,Different,cell,observed,found,enhance,osteocalcin,secretion,while,upregulate,osteopontin,human,osteoblasts,promoted,osteogenesis,but,degraded,earlier,than,Our,findings,suggest,provide,yet,comparable,solutions,optimizing,resorption,balance,These,possess,ideal,potential,repairing

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