The replication of DNA is a complex biological process that is essential for life.Bacterial DNA replication is initiated at genomic loci referred to as replication origins(oriCs).Integrating the Z-curve method,DnaA box distribution,and comparative genomic analysis,we developed a web server to predict bacterial oriCs in 2008 called Ori-Finder,which is helpful to clarify the character-istics of bacterial oriCs.The oriCs of hundreds of sequenced bacterial genomes have been annotated in the genome reports using Ori-Finder and the predicted results have been deposited in DoriC,a manually curated database of oriCs.This has facilitated large-scale data mining of functional ele-ments in oriCs and strand-biased analysis.Here,we describe Ori-Finder 2022 with updated predic-tion framework,interactive visualization module,new analysis module,and user-friendly interface.More species-specific indicator genes and functional elements of oriCs are integrated into the updated framework,which has also been redesigned to predict oriCs in draft genomes.The inter-active visualization module displays more genomic information related to oriCs and their functional elements.The analysis module includes regulatory protein annotation,repeat sequence discovery,homologous oriC search,and strand-biased analyses.The redesigned interface provides additional customization options for oriC prediction.Ori-Finder 2022 is freely available at .

Mei-Jing Dong;Hao Luo;Feng Gao

Department of Physics,School of Science,Tianjin University,Tianjin 300072,China;Frontiers Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering(Ministry of Education),Tianjin University,Tianjin 300072,China;SynBio Research Platform,Collaborative Innovation Center of Chemical Science and Engineering(Tianjin),Tianjin 300072,China

基因组蛋白质组与生物信息学报（英文版）

[1]Mei-Jing Dong;Hao Luo;Feng Gao-.Ori-Finder 2022:A Comprehensive Web Server for Prediction and Analysis of Bacterial Replication Origins)[J].基因组蛋白质组与生物信息学报（英文版）,2022(06):1207-1213

oriCs,DnaA,DoriC,redesigned,oriC

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