Background:Exploring natural sources to identify new active ingredients for cosmeceutical and pharmaceutical applications is of interest to many researchers.Methods:Flavonoid-rich extracts from dark Ficus carica peels were investigated in the present study.Extracts were prepared from the peels of four dark cultivars(Bakor Noir,Bouankik,Azenjer and Tazegaght)using acidified methanol.The antimicrobial activity of the extracts was evaluated by the agar well diffusion assay followed by the minimal inhibitory concentration determination using the microdilution method against Gram-positive and Gram-negative pathogenic bacteria and two fungi.Cosmeceutical properties were evaluated by measuring antioxidant activity,ultraviolet absorption characteristics and sun protection factor values.The antioxidant activity of the extracts was measured using a β-carotene-linoleic acid system.The toxicity of the extracts was assessed using a brine shrimp lethality assay.Results:The extracts contained high amounts of phenolic compounds(3.85-8.63 g/100 g),mainly flavonoids(up to 5 g/100 g).Multiple antibacterial activities were recorded against Gram-positive and Gram-negative bacteria,with the best actions against Bacillus subtilis(minimal inhibitory concentration=156.25 μg/mL),Staphylococcus aureus and Pseudomonas aeruginosa(minimal inhibitory concentration=312.5 μg/mL),but no antifungal activity was recorded.Azenjer extract showed high inhibition of lipid oxidation evaluated by the β-carotene bleaching assay with a half-maximal inhibitory concentration of 88.84 μg/mL.All extracts showed absorption peaks in the ultraviolet range and Azenjer and Tazegaght extracts at 25 mg/mL presented sun protection factor values＞15.The brine shrimp toxicity assay results revealed lethal concentration of 50％values greater than 2,000μg/mL,indicating the safety of the extracts.Conclusion:Therefore,Ficus carica peel extracts could be considered original ingredients with potential applications in the formulation of antibacterial drugs and skincare products.

Leila Meziant;Mostapha Bachir-Bey;Malika Boutiche;Lynda Gali;Assia Ikhlef;Hayette Louaileche

Laboratory of Applied Biochemistry,Faculty of Nature and Life Sciences,University of Bejaia,Bejaia 06000,Algeria;Food Biotechnology Division,Biotechnology Research Center,Constantine 25001,Algeria;Biotechnology and Health Division,Biotechnology Research Center,Constantine 25001,Algeria

传统医学研究（英文版）

[1]Leila Meziant;Mostapha Bachir-Bey;Malika Boutiche;Lynda Gali;Assia Ikhlef;Hayette Louaileche-.Assessment of flavonoid-rich extracts from dark peels of Ficus carica L.fruits for cosmeceutical and antimicrobial applications)[J].传统医学研究（英文版）,2022(06):35-47

cosmeceutical,Bakor,Bouankik,Azenjer,Tazegaght,acidified,microdilution,Cosmeceutical

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