Background: Sepsis-induced coagulopathy and multiple organ dysfunction syndromes are the leading causes of death in patients with sepsis. Qingwen Baidu decoction (QWBD) can effectively improve the clinical manifestations of sepsis and ease inflammation, but its effects on coagulation functions and multiple organ injuries remain unclear. Methods: 100 healthy, male Sprague-Dawley rats were randomly divided into the sham group, the cecal ligation and puncture (CLP) group, the low-dose QWBD group, and the high-dose QWBD group, with 25 rats in each group. The sepsis model was established using CLP. Blood was collected to measure platelet count, serum creatinine (Cr), blood urea nitrogen (BUN), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) levels, as well as coagulation function. The total protein in bronchoalveolar lavage fluid (BALF) was determined in each group of rats. The lung, liver, and kidney tissues were harvested, and statistics were calculated on the wet-to-dry (W/D) weight ratio. Changes in histopathology and thrombin level were evaluated in each group. The remaining ten rats in each group were observed daily to record the number of surviving rats. Such observation was made consecutively for 7 days to calculate survival rates. Results: After model establishment, ALT, AST, Cr, and BUN levels were significantly elevated (P＜0.01)). The BALF protein content and lung W/D weight ratio were significantly increased (P ＜ 0.01). Furthermore, the survival rate of rats was significantly reduced in the CLP group compared with the sham group. After the treatment, rats in the high-dose QWBD group had lower ALT (P ＜0.05), AST (P＜0.01), Cr (P＜0.05), BUN (P＜0.01) levels, lower BALF protein content (P＜0.05) and lower lung W/D weight ratio (P ＜ 0.01) than the CLP group. However, rats in the high-dose QWBD group had significantly better pathological changes in the lung, liver, and kidney compared to the sham group. After the treatment, the platelet level in the peripheral blood was elevated (P＜0.05) and both activated partial thromboplastin time and prothrombin time were significantly shortened (P＜0.01). The fibrinogen level was significantly increased (P＜ 0.01). Finally, thrombin positive expression areas in the lung, liver, and kidney were significantly decreased in the high-dose QWBD group. Conclusion: QWBD can improve coagulation disorders caused by sepsis and has a protective effect on multiple organ injuries in rats.

Bei-Tian Jia;Yu-Lin Wu;Jia-Bao Liao;Wen-Ju He;Dong-Qiang Wang;Feng Chen;Qing-Yun Zhao;Cui-Han Wang;Jun-Li Guo;Yu-Hong Bian;Huan-Tian Cui

Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China;Tianjin Medical University Cancer Institute and Hospital,Tianjin 300060,China;Jiaxing Hospital of Traditional Chinese Medicine,Zhejiang 314033,China;Tianjin First Central Hospital,Tianjin 300190,China;Taian Municipal Hospital,Shandong 271099,China;Tianjin Hospital of Integration of Traditional Chinese and Western Medicine/Nankai Hospital,Tianjin 300102,China;Hebei Hospital of Traditional Chinese Medicine,Hebei 050013,China;Shandong University,Shandong 301600,China

传统医学研究（英文版）

[1]Bei-Tian Jia;Yu-Lin Wu;Jia-Bao Liao;Wen-Ju He;Dong-Qiang Wang;Feng Chen;Qing-Yun Zhao;Cui-Han Wang;Jun-Li Guo;Yu-Hong Bian;Huan-Tian Cui-.Effects of Qingwen Baidu decoction on coagulation and multiple organ injury in rat models of sepsis)[J].传统医学研究（英文版）,2022(03):81-89

QWBD

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