Objective:To investigate the role of Portulaca oleracea(POL)in promoting revascularization and re-epithelization as well as inhibiting iron aggregation and inflammation of deep tissue pressure injury(DTPI).Methods:The hydroalcoholic extract of POL(P)and aqueous phase fraction of POL(PD)were prepared based on maceration and liquid-liquid extraction.The number of new blood vessels and VEGF-A expres-sion level were assessed using H&E stain and Western blot on injured muscle to examine the role of POL different extracts in vascularization.The iron distribution and total elemental iron of injured muscle were detected using laser ablation inductively coupled plasma mass spectrometry(ICP-MS)and Perls'staining to determine whether POL extracts can inhibit the iron accumulation.Besides,the ability of POL extracts to promote wound healing by combining re-epithelization time,inflammation degree and collagen deposition area were comprehensively evaluated.Results:In vitro,we observed a significant increase in HUVEC cell viability,migration rate and the number of the tube after P and PD treatment(P＜0.05).In vivo,administration of P and PD impacted vasculariza-tion and iron accumulation on injured tissue,evident from more new blood vessels,higher expression of VEGF-A and decreased muscle iron concentration of treatment groups compared with no-treatment groups(P＜0.05).Besides,shorter re-epithelization time,reduced inflammatory infiltration and distinct collagen deposition were associated with administration of P and PD(P＜0.05).Conclusion:POL extract administration groups have high-quality wound healing,which is associated with increased new blood vessels,collagen deposition and re-epithelization,along with decreased iron accumulation and inflammatory infiltration.Our results suggest that that POL extract is beneficial to repair injured muscle after ischemia-reperfusion,highlighting the potential of POL in the DTPI treatment.

Jinglin Guo;Juan Peng;Jing Han;Ke Wang;Ruijuan Si;Hui Shan;Xiaoying Wang;Ju Zhang

School of Nursing,Qingdao University,Qingdao 266071,China;Surgery of Traditional Chinese Medicine,Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine,Tianjin 300150,China;School of Pharmacy,Qingdao University,Qingdao 266071,China

中草药（英文版）

[1]Jinglin Guo;Juan Peng;Jing Han;Ke Wang;Ruijuan Si;Hui Shan;Xiaoying Wang;Ju Zhang-.Extracts of Portulaca oleracea promote wound healing by enhancing angiology regeneration and inhibiting iron accumulation in mice)[J].中草药（英文版）,2022(02):263-272

angiology,epithelization,DTPI,maceration,Perls,vasculariza

Extracts,Portulaca,oleracea,promote,wound,healing,by,enhancing,regeneration,inhibiting,iron,accumulation,mice,Objective,To,investigate,role,POL,promoting,revascularization,well,aggregation,inflammation,deep,tissue,pressure,injury,Methods,hydroalcoholic,aqueous,phase,fraction,were,prepared,liquid,extraction,number,new,blood,vessels,VEGF,level,assessed,using,blot,injured,muscle,examine,different,extracts,distribution,total,elemental,detected,laser,ablation,inductively,coupled,plasma,mass,spectrometry,ICP,staining,determine,whether,Besides,combining,degree,collagen,deposition,area,comprehensively,evaluated,Results,In,vitro,observed,significant,HUVEC,cell,viability,migration,rate,tube,after,treatment,vivo,administration,impacted,evident,from,more,higher,expression,decreased,concentration,groups,compared,no,shorter,reduced,inflammatory,infiltration,distinct,associated,Conclusion,have,quality,which,increased,along,Our,results,suggest,that,beneficial,repair,ischemia,reperfusion,highlighting,potential

0.443435