典型文献
Application of a ddRT-PCR to quantify seasonal influenza virus for viral isolation
文献摘要：
Viral isolation in cell cultures has been regarded for decades as the "gold standard" for the laboratory diagnosis of influenza viral infections. Not all viral strains could be isolated from clinical samples. This study aimed to quantify the viral load in the samples before isolation to save working time and improve working efficiency. Four hundred samples from patients with influenza-like cases were confirmed pdmH1N1 positive (200 cases) and B Victoria (BV) positive (200 cases) by whole-genome sequencing and analyzed by ddPCR for viral load in samples before isolation, and isolation results were verified by hemagglutination (HA) assay and hemagglutination-inhibition (HI) tests. Probit regression analysis was used to calculate the isolation viral load limit with a 95% probability level by SPSS 19.0 software. The results showed that the isolation limit of viral load was 4.9 × 10
4 (95% CI: 2.5 × 10
4-9.0 × 10
4) copies/mL for pdmH1N1 and 1.9 × 10
4 (95% CI: 7.8 × 10
3-3.6 × 10
4) copies/mL for BV. The isolation rate of clinical samples is positively correlated with the viral load in clinical samples, which can be used for viral culture, providing important guidance for daily work.
文献关键词：
Influenza virus;ddRT-PCR;Viral load;Viral isolation
中图分类号：
作者姓名：
Liu Yimeng;Zhao Jiachen;Peng Xiaomin;Lu Guilan;Shi Weixian;Feng Zhaomin;Xu Hui;Cui Shujuan;Pan Yang;Zhang Daitao;Yang Peng;Wang Quanyi
作者机构：
Institute for Infectious Disease and Endemic Disease Control, Beijing Center for Disease Prevention and Control, Beijing 100013, China
文献出处：
引用格式：
[1]Liu Yimeng;Zhao Jiachen;Peng Xiaomin;Lu Guilan;Shi Weixian;Feng Zhaomin;Xu Hui;Cui Shujuan;Pan Yang;Zhang Daitao;Yang Peng;Wang Quanyi-.Application of a ddRT-PCR to quantify seasonal influenza virus for viral isolation)[J].生物安全与健康 （英文）,2022(05):299-302
A类：
ddRT,pdmH1N1
B类：
Application,quantify,seasonal,influenza,virus,viral,isolation,Viral,cell,cultures,has,been,regarded,decades,gold,standard,laboratory,diagnosis,infections,Not,all,strains,could,isolated,from,clinical,samples,This,study,aimed,load,before,save,working,improve,efficiency,Four,hundred,patients,like,cases,were,confirmed,Victoria,BV,by,whole,genome,sequencing,analyzed,ddPCR,results,verified,hemagglutination,HA,assay,inhibition,HI,tests,Probit,regression,analysis,was,used,calculate,limit,probability,level,software,showed,that,10
,copies,rate,positively,correlated,which,can,providing,important,guidance,daily,Influenza
AB值：
0.468265
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机标中图分类号，由域田数据科技根据网络公开资料自动分析生成，仅供学习研究参考。
