OBJECTIVE:To observe the anticancer effects of the granular preparation of Tenglong Buzhong decoction(藤龙补中汤,TBD),i.e Tenglong Buzhong granules(藤龙补中颗粒,TBG),in human SW620 colon cancer.METHODS:BALB/c nude mice were subcutaneously transplanted with SW620 cells,and treated with TBG(2.56 g/kg,once per day)and/or 5-Fu(104 mg/kg,once per week)for 21 d.Apoptosis,Caspase activities and cellular senescence were measured by commercial kits.The protein expression and phosphorylation were detected by Western blot or immunohistochemistry.RESULTS:TBG and 5-Fu inhibited tumor growth.The tumor inhibition rate of the TBG,5-Fu,and TBG+5-Fu groups was 42.25％,51.58％,and 76.08％,respectively.Combination of TBG and 5-Fu showed synergetic anti-cancer effects.TBG and 5-Fu induced apoptosis,activated caspase-3,-8,and-9,increased SMAC expression,inhibited XIAP expression.TBG induced cellular senescence,upregulated cyclin-dependent kinase inhibitor 1a(CDKN1a)and cyclin-dependent kinase inhibitor 2a(CDKN2a)expression,and inhibited phosphorylation of retinoblastoma-associated protein(RB)and expression of cyclin E1(CCNE1)and cyclin-dependent kinases(CDK)2.TBG also inhibited angiogenesis accompanied by downregulation of vascular endothelial growth factor(VEGF)and hypoxia-inducible factor-1α(HIF-1α).CONCLUSIONS:TBG inhibits SW620 colon cancer growth,induces apoptosis via SMAC-XIAP-Caspases signaling,induces cellular senescence through CDKN1a/CDKN2a-RB-E2F signaling,inhibits angiogenesis by down-regulation of HIF-1α and VEGF,and enhances the effects of 5-Fu.

LI Miao;ZHENG Jialu;WANG Shuangshuang;CHEN Lei;PENG Xiao;CHEN Jinfang;AN Hongmei;HU Bing

Institute of Traditional Chinese Medicine in Oncology,Longhua Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai 200032,China;Department of Oncology,Longhua Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai 200032,China;Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Department of Science and Technology,Longhua Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai 200032,China

中医杂志（英文版）

[1]LI Miao;ZHENG Jialu;WANG Shuangshuang;CHEN Lei;PENG Xiao;CHEN Jinfang;AN Hongmei;HU Bing-.Tenglong Buzhong granules(藤龙补中颗粒)inhibits the growth of SW620 human colon cancer in vivo)[J].中医杂志（英文版）,2022(05):701-706

Tenglong,Buzhong,TBG+5,CDKN1a,CDKN2a

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