OBJECTIVE:To investigate the efficacy of phospholipid complex of flavonoids from persimmon leaves(PLF-PC)on atherosclerosis,and to study its mechanism behind the action.METHODS:To clarify the constituents of the flavonoids from persimmon leaves(PLF),an ultra-performance liquid chromatography/quadrupole-time-of-flight mass spectrometry method was established.To enhance the anti-atherosclerotic effect of PLF,a newly emerging approach based on the combination of phospholipid complexation technique was employed.PLF-PC was prepared by the solvent-evaporation method then characterized using Fourier transform infrared spectroscopy,Powder X-Ray Diffractometry and Scanning electron microscopy.A model of oxidized low-density lipoprotein-induced injury on human umbilical vein endothelial cells was established to investigate the anti-atherosclerotic effect of PLF-PC versus PLF.The levels of nitric oxide,endothelial nitric oxide synthase,intracellular adhesion molecule-1,reactive oxygen species,superoxide dismutase,tumor necrosis factor-aand nuclear factor-KB were observed via assay kits.RESULTS:A total of 31 compounds were identified in PLF.PLF-PC showed better anti-atherosclerotic power compared with PLF,moreover,enzyme linked immune-osorbent assay analysis showed that the PLF-PC may effect on endothelial dysfunction and atherosclerosis via antioxidant-related mechanisms.CONCLUSIONS:Our findings elucidated that PLF-PC significantly enhanced the PLF's efficacy on atherosclerosis.

CHEN Jinpeng;ZHANG Kexia;LIU Yi;JIN Song;GAI Xiaohong;REN Tao;TIAN Chengwang

State Key Laboratory of Drug Delivery and Pharmacokinetics,Tianjin 300301,China;Tianjin Key Laboratory of TCM quality markers,Tianjin 300301,China;Tianjin Institute of Pharmaceutical Research,Tianjin 300301,China;Tianjin Pharmaceutial Research Institute Co.Ltd.,Tianjin 300462,China;Sinopharm Group Tianjin Co.Ltd.,Tianjin 300040,China

中医杂志（英文版）

[1]CHEN Jinpeng;ZHANG Kexia;LIU Yi;JIN Song;GAI Xiaohong;REN Tao;TIAN Chengwang-.Efficacy of phospholipid complex of flavonoids from persimmon leaves on atherosclerosis,and possible mechanism)[J].中医杂志（英文版）,2022(03):417-425

persimmon,Diffractometry,aand,osorbent

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