典型文献
Bufalin suppresses ovarian cancer cell proliferation via EGFR pathway
文献摘要：
Background::Previous studies have shown that bufalin exerts antitumor effects through various mechanisms. This study aimed to determine the antineoplastic mechanism of bufalin, an extract of traditional Chinese medicine toad venom, in ovarian cancer.Methods::The 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl tetrazolium bromide (MTT), 5-ethynyl-2
′-deoxyuridine (EdU), and colony formation assays were used to investigate the antiproliferative effect of bufalin on the ovarian cancer cell line SK-OV-3. Molecular docking was used to investigate the combination of bufalin and epidermal growth factor receptor (EGFR) protein. Western blotting was performed to detect the expression of EGFR protein and its downstream targets.
Results::Bufalin inhibited the proliferation of SK-OV-3 cells in a dose- and time-dependent manner. Bufalin was confirmed to combine with EGFR protein using molecular docking and downregulate expression of EGFR. Bufalin inhibited phosphorylation of EGFR, protein kinase B (AKT), and extracellular signal-regulated kinase (ERK).Conclusion::Bufalin suppresses the proliferation of ovarian cancer cells through the EGFR/AKT/ERK signaling pathway.
文献关键词：
Bufalin;Ovarian cancer;Epidermal growth factor receptor
中图分类号：
作者姓名：
Dou Lei;Zou Dan;Song Feiran;Jin Yuxin;Li Yin;Zhang Yi
作者机构：
Department of Gynecology, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, China;First Laboratory of Cancer Institute, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, China;Department of Integration of Chinese and Western Medicine, School of Basic Medical Sciences, Peking University, Beijing 100191, China
文献出处：
引用格式：
[1]Dou Lei;Zou Dan;Song Feiran;Jin Yuxin;Li Yin;Zhang Yi-.Bufalin suppresses ovarian cancer cell proliferation via EGFR pathway)[J].中华医学杂志（英文版）,2022(04):456-461
A类：
B类：
Bufalin,suppresses,ovarian,cancer,proliferation,via,EGFR,pathway,Background,Previous,studies,have,shown,that,bufalin,exerts,antitumor,effects,through,various,mechanisms,This,study,aimed,determine,antineoplastic,extract,traditional,Chinese,medicine,toad,venom,Methods,Dimethyl,thiazolyl,diphenyl,tetrazolium,bromide,MTT,ethynyl,2
,deoxyuridine,EdU,colony,formation,assays,were,used,investigate,antiproliferative,line,SK,OV,Molecular,docking,was,combination,epidermal,growth,receptor,protein,blotting,performed,detect,expression,its,downstream,targets,Results,inhibited,cells,dose,dependent,manner,confirmed,combine,using,molecular,downregulate,phosphorylation,kinase,AKT,extracellular,regulated,ERK,Conclusion,signaling,Ovarian,Epidermal
AB值：
0.586399
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