典型文献
SARS-CoV-2 impairs the disassembly of stress granules and promotes ALS-associated amyloid aggregation
文献摘要：
The nucleocapsid(N)protein of SARS-CoV-2 has been reported to have a high ability of liquid-liquid phase separation,which enables its incorporation into stress granules(SGs)of host cells.However,whether SG invasion by N protein occurs in the scenario of SARS-CoV-2 infection is unknow,neither do we know its con-sequence.Here,we used SARS-CoV-2 to infect mam-malian cells and observed the incorporation of N protein into SGs,which resulted in markedly impaired self-dis-assembly but stimulated cell cellular clearance of SGs.NMR experiments further showed that N protein binds to the SG-related amyloid proteins via non-specific tran-sient interactions,which not only expedites the phase transition of these proteins to aberrant amyloid aggre-gation in vitro,but also promotes the aggregation of FUS with ALS-associated P525L mutation in cells.In addition,we found that ACE2 is not necessary for the infection of SARS-CoV-2 to mammalian cells.Our work indicates that SARS-CoV-2 infection can impair the dis-assembly of host SGs and promote the aggregation of SG-related amyloid proteins,which may lead to an increased risk of neurodegeneration.
文献关键词：
中图分类号：
作者姓名：
Yichen Li;Shuaiyao Lu;Jinge Gu;Wencheng Xia;Shengnan Zhang;Shenqing Zhang;Yan Wang;Chong Zhang;Yunpeng Sun;Jian Lei;Cong Liu;Zhaoming Su;Juntao Yang;Xiaozhong Peng;Dan Li
作者机构：
Bio-X Institutes,Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders,Ministry of Education,Shanghai Jiao Tong University,Shanghai 200030,China;School of Life Sciences and Biotechnology,Shanghai Jiao Tong University,Shanghai 200030,China;National Kunming High-level Biosafety Primate Research Center,Institute of Medical Biology,Chinese Academy of Medical Sciences and Peking Union Medical College,Kunming 650031,China;State Key Laboratory of Medical Molecular Biology,Chinese Academy of Medical Sciences,School of Basic Medicine,Peking Union Medical College,Beijing 100005,China;Interdisciplinary Research Center on Biology and Chemistry,Shanghai Institute of Organic Chemistry,Chinese Academy of Sciences,Shanghai 201210,China;University of Chinese Academy of Sciences,Beijing 100049,China;State Key Laboratory of Biotherapy,Department of Geriatrics and National Clinical Research Center for Geriatrics,West China Hospital,Sichuan University,Chengdu 610041,China;State Key Laboratory of Medical Molecular Biology,Department of Biochemistry and Molecular Biology,Institute of Basic Medical Sciences,Chinese Academy of Medical Sciences&Peking Union Medical College,Beijing 100005,China;Bio-X-Renji Hospital Research Center,Renji Hospital,School of Medicine,Shanghai Jiao Tong University,Shanghai 200240,China;Zhangjiang Institute for Advanced Study,Shanghai Jiao Tong University,Shanghai 200240,China
文献出处：
引用格式：
[1]Yichen Li;Shuaiyao Lu;Jinge Gu;Wencheng Xia;Shengnan Zhang;Shenqing Zhang;Yan Wang;Chong Zhang;Yunpeng Sun;Jian Lei;Cong Liu;Zhaoming Su;Juntao Yang;Xiaozhong Peng;Dan Li-.SARS-CoV-2 impairs the disassembly of stress granules and promotes ALS-associated amyloid aggregation)[J].蛋白质与细胞,2022(08):602-614
A类：
unknow,P525L
B类：
SARS,CoV,impairs,disassembly,stress,granules,promotes,ALS,associated,amyloid,aggregation,nucleocapsid,been,reported,have,high,ability,liquid,phase,separation,which,enables,its,incorporation,into,SGs,host,cells,However,whether,invasion,by,occurs,scenario,infection,neither,do,con,sequence,Here,used,observed,resulted,markedly,impaired,self,but,stimulated,cellular,clearance,NMR,experiments,further,showed,that,binds,related,proteins,via,specific,sient,interactions,not,only,expedites,transition,these,aberrant,vitro,also,FUS,mutation,In,addition,found,ACE2,necessary,mammalian,Our,work,indicates,can,may,lead,increased,risk,neurodegeneration
AB值：
0.488156
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