典型文献
Homology-based repair induced by CRISPR-Cas nucleases in mammalian embryo genome editing
文献摘要：
Recent advances in genome editing,especially CRISPR-Cas nucleases,have revolutionized both laboratory research and clinical therapeutics.CRISPR-Cas nucle-ases,together with the DNA damage repair pathway in cells,enable both genetic diversification by classical non-homologous end joining(c-NHEJ)and precise genome modification by homology-based repair(HBR).Genome editing in zygotes is a convenient way to edit the germline,paving the way for animal disease model generation,as well as human embryo genome editing therapy for some life-threatening and incurable dis-eases.HBR efficiency is highly dependent on the DNA donor that is utilized as a repair template.Here,we review recent progress in improving CRISPR-Cas nuclease-induced HBR in mammalian embryos by designing a suitable DNA donor.Moreover,we want to provide a guide for producing animal disease models and correcting genetic mutations through CRISPR-Cas nuclease-induced HBR in mammalian embryos.Finally,we discuss recent developments in precise genome-modification technology based on the CRISPR-Cas system.
文献关键词：
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作者姓名：
Xiya Zhang;Tao Li;Jianping Ou;Junjiu Huang;Puping Liang
作者机构：
Center for Reproductive Medicine,the Third Affiliated Hospital of Sun Yat-sen University,Sun Yat-sen University,Guangzhou 510630,China;MOE Key Laboratory of Gene Function and Regulation,State Key Laboratory of Biocontrol,School of Life Sciences,Sun Yat-sen University,Guangzhou 510275,China;Key Laboratory of Reproductive Medicine of Guangdong Province,the First Affiliated Hospital and School of Life Sciences,Sun Yat-sen University,Guangzhou 510275,China
文献出处：
引用格式：
[1]Xiya Zhang;Tao Li;Jianping Ou;Junjiu Huang;Puping Liang-.Homology-based repair induced by CRISPR-Cas nucleases in mammalian embryo genome editing)[J].蛋白质与细胞,2022(05):316-335
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0.504151
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