Infection is a common problem after implanta-tion,and the currently used Ti-based implants have insuf-ficient antibacterial activities.The newly developed Zr-30Ta and Zr-25Ta-5Ti alloys exhibited good mechanical proper-ties,low elastic moduli,and high corrosion resistance.In this work,their antibacterial activities and antibacterial mecha-nisms were investigated.The surface roughness,wettability,and charge of the alloys were evaluated using a profilometer,drop shape analyzer,and electrokinetic analyzer,respec-tively.The antibacterial activity against Streptococcus mutans and Porphyromonas gingivalis was investigated using the inhibition zone assay,plate count assay,live/dead staining,and scanning electron microscopy(SEM).The reactive oxygen species release(ROS)was evaluated using the 2'7'-dichlorodihydrofluorescein diacetate(DCFH-DA)assay.The results demonstrated that Zr-30Ta and Zr-25Ta-5Ti alloys had favorable smooth,hydrophilic,negatively charged surfaces that decreased bacteria adhesion and pre-vented biofilm formation.Zr-30Ta and Zr-25Ta-5Ti alloys also exhibited a time-dependent decrease in the bacteria adhesion and viability.Zr-30Ta and Zr-25Ta-5Ti alloys were also found to induce direct membrane damage and increase ROS production,resulting in significantly better antibacterial activities than CP-Ti.Zr-30Ta and Zr-25Ta-5Ti alloys exhibited comparable antibacterial activities.This work suggests that the newly developed Zr-30Ta and Zr-25Ta-5Ti alloys have good antibacterial activity against Streptococcus mutans and Porphyromonas gingivalis and are promising candidate materials for dental and orthopedic implants.

Hai-Lin Yang;Akram Nasser Juaim;Ling Zou;Meng-Zhen Zhu;Xiao-Na Chen;Chen-Xu Ma;Xiong-Wen Zhou

State Key Laboratory of Powder Metallurgy,Central South University,Changsha 410083,China;National Clinical Research Center for Geriatric Disorders,Xiangya Hospital,Central South University,Changsha 410008,China;Department of Prosthodontics,Center of Stomatology,Xiangya Hospital,Central South University,Changsha 410008,China;Department of Prosthodontics,Faculty of Dentistry,Thamar University,Dhamar,Yemen;Department of Oral Implantology,Xiangya Stomatological Hospital and School of Stomatology,Central South University,Changsha 410008,China

稀有金属（英文版）

[1]Hai-Lin Yang;Akram Nasser Juaim;Ling Zou;Meng-Zhen Zhu;Xiao-Na Chen;Chen-Xu Ma;Xiong-Wen Zhou-.Antibacterial activity and mechanism of newly developed Zr-30Ta and Zr-25Ta-5Ti alloys against implant-associated infection)[J].稀有金属（英文版）,2022(12):4176-4187

30Ta,25Ta

Antibacterial,activity,mechanism,newly,developed,Zr,5Ti,alloys,against,associated,infection,Infection,common,problem,after,implanta,currently,used,implants,have,insuf,ficient,antibacterial,activities,exhibited,good,mechanical,proper,low,elastic,moduli,high,corrosion,resistance,this,work,their,nisms,were,investigated,roughness,wettability,evaluated,using,profilometer,shape,analyzer,electrokinetic,respec,Streptococcus,mutans,Porphyromonas,gingivalis,was,inhibition,zone,assay,plate,count,live,dead,staining,scanning,electron,microscopy,reactive,oxygen,species,release,ROS,dichlorodihydrofluorescein,diacetate,DCFH,DA,results,demonstrated,that,had,favorable,smooth,hydrophilic,negatively,charged,surfaces,decreased,adhesion,pre,vented,biofilm,formation,also,dependent,viability,found,induce,direct,membrane,damage,increase,production,resulting,significantly,better,than,CP,comparable,This,suggests,are,promising,candidate,materials,dental,orthopedic

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