典型文献
An alternative to MINFLUX that enables nanometer resolution in a confocal microscope
文献摘要：
Localization of single fluorescent emitters is key for physicochemical and biophysical measurements at the nanoscale and beyond ensemble averaging.Examples include single-molecule tracking and super-resolution imaging by single-molecule localization microscopy.Among the numerous localization methods available,MINFLUX outstands for achieving a～10-fold improvement in resolution over wide-field camera-based approaches,reaching the molecular scale at moderate photon counts.Widespread application of MINFLUX and related methods has been hindered by the technical complexity of the setups.Here,we present RASTMIN,a single-molecule localization method based on raster scanning a light pattern comprising a minimum of intensity.RASTMIN delivers～1-2nm localization precision with usual fluorophores and is easily implementable on a standard confocal microscope with few modifications.We demonstrate the performance of RASTMIN in localization of single molecules and super-resolution imaging of DNA origami structures.
文献关键词：
中图分类号：
作者姓名：
Luciano A.Masullo;Alan M.Szalai;Lucía F.Lopez;Mauricio Pilo-Pais;Guillermo P.Acuna;Fernando D.Stefani
作者机构：
Centro de Investigaciones en Bionanociencias(CIBION),Consejo Nacional de Investigaciones Científicas y Técnicas(CONICET),Godoy Cruz 2390,C1425FQD Ciudad Autónoma de Buenos Aires,Buenos Aires,Argentina;Departamento de Física,Facultad de Ciencias Exactas y Naturales,Universidad de Buenos Aires,Güiraldes 2620,C1428EHA Ciudad Autónoma de Buenos Aires,Buenos Aires,Argentina;Department of Physics,University of Fribourg,Chemin du Musée 3,Fribourg CH-1700,Switzerland
文献出处：
引用格式：
[1]Luciano A.Masullo;Alan M.Szalai;Lucía F.Lopez;Mauricio Pilo-Pais;Guillermo P.Acuna;Fernando D.Stefani-.An alternative to MINFLUX that enables nanometer resolution in a confocal microscope)[J].光：科学与应用（英文版）,2022(11):2077-2085
A类：
MINFLUX,outstands,RASTMIN
B类：
An,alternative,that,enables,nanometer,resolution,confocal,microscope,Localization,single,fluorescent,emitters,key,physicochemical,biophysical,measurements,nanoscale,beyond,ensemble,averaging,Examples,include,tracking,super,imaging,by,localization,microscopy,Among,numerous,methods,available,achieving,fold,improvement,over,wide,field,camera,approaches,reaching,molecular,moderate,photon,counts,Widespread,application,related,has,been,hindered,technical,complexity,setups,Here,we,present,raster,scanning,light,pattern,comprising,minimum,intensity,delivers,2nm,precision,usual,fluorophores,easily,implementable,standard,few,modifications,We,demonstrate,performance,molecules,origami,structures
AB值：
0.624052
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