典型文献
In-situ hydrophobic environment triggering reactive fluorescence probe to real-time monitor mitochondrial DNA damage
文献摘要：
Mitochondrial DNA has a special structure that is prone to damage resulting in many serious diseases,such as genetic diseases and cancers.Therefore,the rapid and specific monitoring of mitochondrial DNA damage is urgently needed for biological recognition.Herein,we constructed an in situ hydrophobic environment-triggering reactive fluorescence probe named MBI-CN.The fluor-ophore was 2-styrene-1H-benzo[d]imidazole,and malo-nonitrile was introduced as a core into a molecule to initiate the hydrolysis reaction in the specific environment containing damaged mitochondrial DNA.In this design,MBI-CN conjugates to mitochondrial DNA without causing additional damages.Thus,MBI-CN can be hydrolyzed to generate MBI-CHO in an in situ hydro-phobic environment with mitochondrial DNA damage.Meanwhile,MBI-CHO immediately emitted a significative fluorescence signal changes at 437 and 553 nm within 25 s for the damaged mitochondria DNA.Give that the specific and rapid response of MBI-CN does not cause additional damages to mitochondrial DNA,it is a potentially effective detection tool for the real-time monitoring of mitochon-drial DNA damage during cell apoptosis and initial assessment of cell apoptosis.
文献关键词：
中图分类号：
作者姓名：
Beidou Feng;Huiyu Niu;Hongchen Zhai;Congcong Shen;Hua Zhang
作者机构：
School of Chemistry and Chemical Engineering,Henan Normal University,Xinxiang 453007,China
文献出处：
引用格式：
[1]Beidou Feng;Huiyu Niu;Hongchen Zhai;Congcong Shen;Hua Zhang-.In-situ hydrophobic environment triggering reactive fluorescence probe to real-time monitor mitochondrial DNA damage)[J].化学科学与工程前沿,2022(01):92-102
A类：
fluor,ophore,malo,nonitrile,significative
B类：
In,situ,hydrophobic,environment,triggering,reactive,fluorescence,probe,real,mitochondrial,Mitochondrial,has,special,structure,that,prone,resulting,many,serious,diseases,such,genetic,cancers,Therefore,rapid,specific,monitoring,urgently,needed,biological,recognition,Herein,we,constructed,named,MBI,CN,was,styrene,1H,benzo,imidazole,introduced,core,into,molecule,initiate,hydrolysis,reaction,containing,damaged,this,design,conjugates,without,causing,additional,damages,Thus,hydrolyzed,generate,CHO,Meanwhile,immediately,emitted,signal,changes,within,Give,response,does,not,cause,potentially,effective,detection,tool,during,cell,apoptosis,initial,assessment
AB值：
0.479221
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