Penicillium oxalicum SL2(SL2)is a previously screened Pb-tolerant fungus that can promote crops growth.The relationship between SL2 colonization and Pb immobilization was stud-ied to provide a theoretical basis for microbial remediation of Pb-contaminated paddy soil.In this study,green fluorescent protein(GFP)labeled SL2 was inoculated into different Pb-contaminated paddy soils(S1-S6).The Pb extracted from the soil by HNO3,EDTA and CaCl2 were used to characterize the available Pb.The results showed that the colonization of SL2 was divided into lag phase(0-7 days),growth phase(7-30 days),and mortality phase(30-90 days).SL2 colonized well in sandy soils rich in clay and total phosphorus with initial pH of 4.5-7.0.In addition,SL2 increased soil pH and decreased soil Eh,which was beneficial to immobilize Pb.In different soils,the highest percentages of CaCl2-Pb,EDTA-Pb,and HNO3-Pb immobilized by SL2 were 34.34％-40.53％,17.05％-20.111％,and 7.39％-15.62％,respectively.Pearson correlation analysis showed that the percentages of CaCl2-Pb and EDTA-Pb immo-bilized by SL2 were significantly positively correlated with the number of SL2 during the growth phase.SL2 mainly immobilized Pb in the growth phase and a higher peak number of SL2 was beneficial to the immobilization of Pb.

Yu Gao;Xiaohan Jiang;Hanxin Wu;Jianhao Tong;Xinyue Ren;Jiayu Ren;Qianhua Wu;Jien Ye;Chunhui Li;Jiyan Shi

Department of Environmental Engineering,College of Environmental and Resource Science,Zhejiang University,Hangzhou 310058,China;MOE Key Laboratory of Environmental Remediation and Ecological Health,College of Environmental and Resource Science,Zhejiang University,Hangzhou 310058,China

环境科学学报（英文版）

[1]Yu Gao;Xiaohan Jiang;Hanxin Wu;Jianhao Tong;Xinyue Ren;Jiayu Ren;Qianhua Wu;Jien Ye;Chunhui Li;Jiyan Shi-.Colonization of Penicillium oxalicum SL2 in Pb-contaminated paddy soil and its immobilization effect on soil Pb)[J].环境科学学报（英文版）,2022(10):53-62

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