Amino acids(AAs)are prevalent in source water,particularly during spring run-off.Mon-itoring of amino acids in source water is desirable for water treatment plants(WTP)to indicate changes in source water quality.The objective of this study was to establish an-alytical procedures for reliable monitoring of amino acids in source water.Therefore,we examined two different methods,large volume inject(LVI)and solid phase extraction(SPE),for sample preparation prior to HILIC-MS/MS.The LVI-HILIC-MS/MS method can provide fast and sensitive detection for clean samples,but suffers from matrix effects,resulting in irreproducible separation and shortening column lifetime.We have demonstrated that SPE was necessary prior to HILIC-MS/MS to achieve reproducible and reliable quantification of AAs in source water.A natural heterocyclic amine 1-methyl-1,2,3,4-tetrahydro-β-carboline-3-carboxylic acid(MTCCA)was also included in the method to indicate changes in other natural nitrogenous compounds in source water.The SPE-HILIC-MS/MS method was able to achieve limits of detection from 2.6-3400 ng/L for the amino acids and MTCCA with RSDs(n=3)of 1.1％-4.8％.As well,retention times(RT)of the analytes were reproducible with vari-ation less than 0.01 min(n=3)through the entire project.We further applied the SPE-HILIC-MS/MS method to determine AAs in authentic source water samples collected from two drinking water treatment plants(WTPs)during the 2021 spring run-off season.The results support that the SPE-HILIC-MS/MS method does not require derivatization and can provide reliable,accurate,and robust analysis of AAs and MTCCA in source water,supporting future monitoring of source water quality.

Junlang Qiu;Caley Craven;Nicholas Wawryk;Kristin Carroll;Xing-Fang Li

Division ofAnalytical and Environmental Toxicology,Department of Laboratory Medicine and Pathology,University of Alberta,Edmonton,T6G 2G3,Canada;School of Environmental Science and Engineering,Sun Yat-sen University,Guangzhou 510006,China

环境科学学报（英文版）

[1]Junlang Qiu;Caley Craven;Nicholas Wawryk;Kristin Carroll;Xing-Fang Li-.Integration of solid phase extraction with HILIC-MS/MS for analysis of free amino acids in source water)[J].环境科学学报（英文版）,2022(07):190-196

alytical,irreproducible,MTCCA,WTPs

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