Particulate matter emissions(PM10)from the combustion,in a residential stove,of two com-mercial brands of certified(ENplus A1)pellets,a non-certified brand and laboratory made pellets of acacia were tested for their ability to induce ecotoxic,cytotoxic,and mutagenic re-sponses in unicellular organisms and a human cell line.Ecotoxicity was evaluated through the Vibrio fischeri bioluminescence inhibition assay.Moreover,cytotoxicity was assessed at two time points(24-and 48-hr)through two complementary techniques in order to evaluate the cellular metabolic activity and membrane integrity of human lung epithelial cells A549.The Ames test using two Salmonella typhimurium strains(TA100 and TA98)was employed to assess the mutagenic potential of the polycyclic aromatic hydrocarbon fraction extracted from the PM10 samples.Results obtained with the bioluminescent bacteria indicated that only particles from the combustion of acacia pellets were toxic.All samples induced im-pairment on the A549 cells metabolic activity,while no significant release of lactate dehy-drogenase was recorded.PM10 emissions from acacia pellets were the most cytotoxic,while samples from both certified pellets evoked significant cytotoxicity at lower doses.Cytotox-icity time-dependency was only observed for PM10 from the combustion of acacia pellets and one of the brands of certified pellets.Mutagenic activity was not detected in both S.typhi murium strains.This study emphasises the role of the raw material for pellet manu-facturing on the toxicological profile of PM emissions.Alternative raw materials should be deeply investigated before their use in pelletisation and combustion in residential appli-ances.

Estela D.Vicente;Daniela Figueiredo;Cátia Gon?alves;Isabel Lopes;Helena Oliveira;Nora Kováts;Teresa Pinheiro;Célia A.Alves

Department of Environment and Planning and CESAM-Centre for Environmental and Marine Studies,University of Aveiro,3810-193 Aveiro,Portugal;Department of Biology and CESAM-Centre for Environmental and Marine Studies,University of Aveiro,3810-193 Aveiro,Portugal;Centre of Natural Environmental Sciences,University of Pannonia,Egyetem str.10,8200 Veszprém,Hungary;Instituto de Bioengenharia e Biociências,Departamento de Engenharia e Ciências Nucleares,Instituto Superior Técnico,Universidade de Lisboa,Av.Rovisco Pais 1,1049-001 Lisboa,Portugal

环境科学学报（英文版）

[1]Estela D.Vicente;Daniela Figueiredo;Cátia Gon?alves;Isabel Lopes;Helena Oliveira;Nora Kováts;Teresa Pinheiro;Célia A.Alves-.In vitro toxicity of particulate matter emissions from residential pellet combustion)[J].环境科学学报（英文版）,2022(05):215-226

stove,ENplus,ecotoxic,Ecotoxicity,bioluminescent,Cytotox,Mutagenic,murium,emphasises,pelletisation

In,vitro,particulate,matter,emissions,from,residential,combustion,Particulate,PM10,two,mercial,brands,certified,pellets,laboratory,made,acacia,were,tested,their,ability,mutagenic,sponses,unicellular,organisms,human,line,was,evaluated,through,Vibrio,fischeri,bioluminescence,inhibition,assay,Moreover,cytotoxicity,assessed,points,complementary,techniques,order,metabolic,activity,membrane,integrity,lung,epithelial,cells,A549,Ames,using,Salmonella,typhimurium,strains,TA100,TA98,employed,potential,polycyclic,aromatic,hydrocarbon,fraction,extracted,samples,Results,obtained,bacteria,indicated,that,only,particles,All,induced,pairment,while,significant,release,lactate,dehy,drogenase,recorded,most,both,evoked,lower,doses,dependency,observed,not,detected,This,study,role,raw,manu,facturing,toxicological,profile,Alternative,materials,should,deeply,investigated,before,use,appli,ances

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