Objective:To investigate the changes in the hypothalamic metabolites of lipopolysaccharide(LPS)febrile young rabbits after the treatment with pediatric tuina.Methods:A total of 30 young rabbits were randomly assigned into three groups:the normal group,the model group,and the tuina group.Both the model group and the tuina group were injected intrave-nously with LPS."Six antipyretic manipulations"(pushing Tianmen,pushing Kangong,kneading Taiyang,kneading Erhougaogu,clearing Tianheshui,and pushing Jizhu)were administered 1 h after the LPS in-jection in the tuina group.The rectal temperatures of the young rabbits were monitored during the experiment to explore the antipyretic effect.Three hours after the injection,the content of interleukin-1 β(IL-1β),tumor necrosis factor(TNF)-α,and prostaglandin E2(PGE2)in the serum was detected.In addition,liquid chromatography-mass spectrometry(LC-MS)was used for the hypothalamus metabolomics.Results:Compared with the model group,the rectal temperature of the tuina group was decreased at 2 h and 3 h after the LPS injection(P=.04,P=.03,respectively),and the content of IL-1β,TNF-α,and PGE2 was decreased(P=.03,P=.003,and P=.008,respectively).The metabolomics results showed that there were 23 potential biomarkers after the tuina intervention,enriching 27 pathways.Lipid metabolites,especially glycerophospholipids,were a majority of the altered metabolites.The primary metabolic pathways affected by tuina included the arachidonic acid metabolism,the GABAergic synapse,D-gluta-mine and D-glutamate metabolism,and the glutamatergic synapse.Conclusion:Pediatric tuina reduced the temperature of the febrile rabbits and downregulated the expression of IL-1β,TNF-α,and PGE2,and the antipyretic mechanism may be related to changes in metabolites and metabolic pathways in the hypothalamus.

Zhifeng Liu;Yi Jiao;Tianyuan Yu;Yingqi Zhang;Di Liu;Hourong Wang;Yajing Xu;Qian Guan;Taotao Lv;Jian Shu

School of Acupuncture-Moxibustion and Tuina,Beijing University of Chinese Medicine,Beijing,102488,China;Department of Acupuncture,Dongfang Hospital Beijing University of Chinese Medicine,Beijing,100078,China;Shu Acupuncture Clinic,Fayetteville,NY,13066,USA

中医科学杂志（英文）

[1]Zhifeng Liu;Yi Jiao;Tianyuan Yu;Yingqi Zhang;Di Liu;Hourong Wang;Yajing Xu;Qian Guan;Taotao Lv;Jian Shu-.Effect of pediatric tuina on hypothalamic metabolites in young rabbits using liquid chromatography-mass spectrometry)[J].中医科学杂志（英文）,2022(02):188-196

tuina,Tianmen,Kangong,kneading,Taiyang,Erhougaogu,Tianheshui,Jizhu

Effect,pediatric,hypothalamic,metabolites,young,rabbits,using,liquid,chromatography,mass,spectrometry,Objective,To,investigate,changes,lipopolysaccharide,LPS,febrile,after,treatment,Methods,total,were,randomly,assigned,into,three,groups,normal,model,Both,injected,intrave,nously,Six,antipyretic,manipulations,pushing,clearing,administered,rectal,temperatures,monitored,during,experiment,explore,effect,Three,hours,injection,content,interleukin,tumor,necrosis,prostaglandin,PGE2,serum,was,detected,In,addition,LC,used,hypothalamus,metabolomics,Results,Compared,decreased,respectively,results,showed,that,there,potential,biomarkers,intervention,enriching,pathways,Lipid,especially,glycerophospholipids,majority,altered,primary,metabolic,affected,by,included,arachidonic,acid,metabolism,GABAergic,synapse,mine,glutamatergic,Conclusion,Pediatric,reduced,downregulated,expression,mechanism,may,be,related

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