Processing bioactive peptides from natural sources using electrodialysis with ultrafiltration membranes (EDUF) have gained attention since it can fractionate in terms of their charge and molecular weight. Quinoa is a pseudo-cereal highlighted by its high protein content, amino acid profile and adapting growing conditions. The present work aimed at the production of quinoa peptides through fractionation using EDUF and to test the fractions according to antihypertensive and antidiabetic activity. Experimental data showed the production of peptides ranging between 0.4 and 1.5 kDa. Cationic (CQPF) (3.01％), anionic (AQPF) (1.18％) and the electrically neutral fraction quinoa protein hydrolysate (QPH)-EDUF (～95％) were obtained. In-vitro studies showed the highest glucose uptake modulation in L6 cell skeletal myoblasts in presence of QPH-EDUF and AQPF (17％ and 11％) indicating potential antidiabetic activity. The antihypertensive effect studied in-vivo in spontaneously hypertensive rats (SHR), showed a decrease in systolic blood pressure in presence of the fractionated peptides, being 100 mg/kg a dose comparable to Captopril (positive control). These results contribute to the current knowledge of bioactive peptides from quinoa by reporting the relevance of EDUF as tool to produce selected peptide fractions. Nevertheless, further characterization is needed towards peptide sequencing, their respective role in the metabolism and scaling-up production using EDUF.

Adrián González-Mu?oz;Marion Valle;Rotimi E.Aluko;Laurent Bazinet;Javier Enrione

Biopolymer Research and Engineering Lab(BiopREL),School of Nutrition and Dietetics,Faculty of Medicine,Universidad de los Andes,Monse?orálvaro del Portillo 12455,Chile;Centre for Biomedical Research and Innovation(CIIB),Universidad de los Andes,Chile,Monse?orálvaro del Portillo 12455,Chile;Institute of Nutrition and Functional Foods(INAF),UniversitéLaval,Québec QC G1V 0A6,Canada;Department of Anatomy and Physiology,Faculty of Medicine,Laval Hospital Research Center,UniversitéLaval,Québec QC G1V 4G5,Canada;Department of Food and Human Nutritional Sciences,University of Manitoba,Winnipeg MB R3T 2N2,Canada;Department of Food Sciences,Laboratoire de Transformation Alimentaire et ProcédésélectroMembranaire(LTAPEM,Laboratory ofFood Processing and Electro-Membrane Processes),UniversitéLaval,Québec QC G1V 0A6,Canada

食品科学与人类健康（英文）

[1]Adrián González-Mu?oz;Marion Valle;Rotimi E.Aluko;Laurent Bazinet;Javier Enrione-.Production of antihypertensive and antidiabetic peptide fractions from quinoa (Chenopodium quinoa Willd.) by electrodialysis with ultrafiltration membranes)[J].食品科学与人类健康（英文）,2022(06):1650-1659

EDUF,CQPF,AQPF,QPH

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