典型文献
Sensitive detection of microRNAs using polyadenine-mediated fluorescent spherical nucleic acids and a microfluidic electrokinetic signal amplification chip
文献摘要：
The identification of tumor-related microRNAs(miRNAs)exhibits excellent promise for the early diag-nosis of cancer and other bioanalytical applications.Therefore,we developed a sensitive and efficient biosensor using polyadenine(polyA)-mediated fluorescent spherical nucleic acid(FSNA)for miRNA analysis based on strand displacement reactions on gold nanoparticle(AuNP)surfaces and electrokinetic signal amplification(ESA)on a microfluidic chip.In this FSNA,polyA-DNA biosensor was anchored on AuNP surfaces via intrinsic affinity between adenine and Au.The upright conformational polyA-DNA recognition block hybridized with 6-carboxyfluorescein-labeled reporter-DNA,resulting in fluores-cence quenching of FSNA probes induced by AuNP-based resonance energy transfer.Reporter DNA was replaced in the presence of target miRNA,leading to the recovery of reporter-DNA fluorescence.Sub-sequently,reporter-DNAs were accumulated and detected in the front of with Nafion membrane in the microchannel by ESA.Our method showed high selectivity and sensitivity with a limit of detection of 1.3 pM.This method could also be used to detect miRNA-21 in human serum and urine samples,with re-coveries of 104.0％-113.3％and 104.9％-108.0％,respectively.Furthermore,we constructed a chip with three parallel channels for the simultaneous detection of multiple tumor-related miRNAs(miRNA-21,miRNA-141,and miRNA-375),which increased the detection efficiency.Our universal method can be applied to other DNA/RNA analyses by altering recognition sequences.
文献关键词：
中图分类号：
作者姓名：
Jun Xu;Qing Tang;Runhui Zhang;Haoyi Chen;Bee Luan Khoo;Xinguo Zhang;Yue Chen;Hong Yan;Jincheng Li;Huaze Shao;Lihong Liu
作者机构：
NMPA Key Laboratory for Research and Evaluation of Drug Metabolism,Guangdong Provincial Key Laboratory of New Drug Screening,School of Pharmaceutical Sciences,Southern Medical University,Guangzhou,510515,China;The Second Clinical Medical School,Southern Medical University,Guangzhou,510515,China;Department of Biomedical Engineering,City University of Hong Kong,Hong Kong,999077,China
文献出处：
引用格式：
[1]Jun Xu;Qing Tang;Runhui Zhang;Haoyi Chen;Bee Luan Khoo;Xinguo Zhang;Yue Chen;Hong Yan;Jincheng Li;Huaze Shao;Lihong Liu-.Sensitive detection of microRNAs using polyadenine-mediated fluorescent spherical nucleic acids and a microfluidic electrokinetic signal amplification chip)[J].药物分析学报（英文）,2022(05):808-813
A类：
polyadenine,FSNA,coveries
B类：
Sensitive,detection,microRNAs,using,mediated,fluorescent,spherical,nucleic,acids,microfluidic,electrokinetic,signal,amplification,chip,identification,tumor,related,miRNAs,exhibits,excellent,promise,early,diag,nosis,cancer,other,bioanalytical,applications,Therefore,developed,sensitive,efficient,biosensor,polyA,analysis,strand,displacement,reactions,gold,nanoparticle,AuNP,surfaces,ESA,In,this,was,anchored,via,intrinsic,affinity,between,upright,conformational,recognition,block,hybridized,carboxyfluorescein,labeled,reporter,resulting,quenching,probes,induced,by,resonance,energy,transfer,Reporter,replaced,presence,target,leading,recovery,fluorescence,Sub,sequently,DNAs,were,accumulated,detected,front,Nafion,membrane,microchannel,Our,method,showed,high,selectivity,sensitivity,limit,pM,This,could,also,used,human,serum,urine,samples,respectively,Furthermore,constructed,three,parallel,channels,simultaneous,multiple,which,increased,efficiency,universal,applied,analyses,altering,sequences
AB值：
0.570551
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