典型文献
Optimization of Three-Dimensional Culture Conditions of HepG2 Cells with Response Surface Methodology Based on the VitroGel System
文献摘要：
Objective This study optimizes three-dimensional (3D) culture conditions of HepG2 using response surface methodology (RSM) based on the VitroGel system to facilitate the cell model in vitro for liver tissues.Method HepG2 cell was 3D cultured on the VitroGel system. Cell viability was detected using Cell Counting Kit-8 (CCK-8) assay of HepG2 lived cell numbers. The proliferation of HepG2 cell and clustering performance was measured via fluorescence staining test. Albumin concentration in the culture medium supernatant as an index of HepG2 cell biological function was measured with ELISA kit. Independent factor tests were conducted with three key factors: inoculated cell concentration, cultured time, and dilution degree of the hydrogel. The preliminary results of independent factor tests were used to determine the levels of factors for RSM. Result The selected optimal culture conditions are as follows: concentration of inoculated cells was 4.44 × 105/mL, culture time was 4.86 days, and hydrogel dilution degree was 1:2.23. The result shows that under optimal conditions, the predicted optical density (OD) value of cell viability was 3.10 and measured 2.978 with a relative error of 3.94％. Conclusion This study serves as a reference for the 3D HepG2 culture and constructs liver tissues in vitro. Additionally, it provides the foundation for repeated dose high-throughput toxicity studies and other scientific research work.
文献关键词：
中图分类号：
作者姓名：
WANG Jing Bo;QIN Wen;YANG Zhuo;SHEN Shi;MA Yan;WANG Li Yuan;ZHUO Qin;GONG Zhao Long;HUO Jun Sheng;CHEN Chen
作者机构：
National Institute for Nutrition and Health,Chinese Centre for Disease Control and Prevention,Beijing 100050,China
文献出处：
引用格式：
[1]WANG Jing Bo;QIN Wen;YANG Zhuo;SHEN Shi;MA Yan;WANG Li Yuan;ZHUO Qin;GONG Zhao Long;HUO Jun Sheng;CHEN Chen-.Optimization of Three-Dimensional Culture Conditions of HepG2 Cells with Response Surface Methodology Based on the VitroGel System)[J].生物医学与环境科学（英文版）,2022(08):688-698
A类：
VitroGel
B类：
Optimization,Three,Dimensional,Culture,Conditions,HepG2,Cells,Response,Surface,Methodology,Based,System,Objective,This,study,optimizes,three,dimensional,conditions,using,response,surface,methodology,RSM,system,facilitate,model,vitro,liver,tissues,was,cultured,viability,detected,Counting,Kit,CCK,assay,lived,numbers,proliferation,clustering,performance,measured,fluorescence,staining,Albumin,concentration,medium,supernatant,biological,function,kit,Independent,tests,were,conducted,key,factors,inoculated,dilution,degree,hydrogel,preliminary,results,independent,used,determine,levels,Result,selected,optimal,are,follows,cells,days,shows,that,under,predicted,optical,density,OD,value,relative,error,Conclusion,serves,reference,constructs,Additionally,provides,foundation,repeated,dose,high,throughput,toxicity,studies,other,scientific,research,work
AB值：
0.53946
相似文献
机标中图分类号，由域田数据科技根据网络公开资料自动分析生成，仅供学习研究参考。
