Knowledge of complete mitochondrial genomes(mitogenomes)can help understand the molecular evolution and phy-logenetic relationships of various species.To date,the phylogenetic status of Leucosiidae within Brachyura remains unresolved be-cause of the limited number of mitogenomes available.In the present study,the complete mitogenome of Myra affinis was sequenced using next-generation sequencing to supplement the limited mitogenome information of Leucosiidae.The 15349 bp-long mitoge-nome includes 13 protein-coding genes(PCGs),22 transfer RNAs(tRNAs),2 ribosomal RNAs(rRNAs),and a putative control re-gion.The overall base composition is 34.5％A,36.1％T,11.5％G,and 17.9％C,with a high AT bias(70.6％).All 13 PCGs start with the standard ATN codon and stop with the TAN codon or incomplete T.Except for tRNA-Ser,all other tRNAs have a typical clover-leaf-like secondary structure.Phylogenetic analyses based on the maximum likelihood and Bayesian inference methods are employed to generate identical phylogenetic topologies,thereby supporting the sister relationship between Leucosiidae and Matutidae for the first time.The monophyly of Eubrachyura is well established,and its sister relationship with Raninoida is strongly supported.The results of this work will not only help achieve a better understanding of the characteristics of the M.affinis mitogenome and the phy-logenetic position of Leucosiidae,but also provide relevant information for further studies on the phylogeny of Brachyura.

ZHANG Ying;MENG Lei;MIAO Zengliang;WEI Liming;LIU Bingjian;LIU Liqin;GONG Li;Lü Zhenming

Engineering Laboratory of Marine Germplasm Resources Exploration and Utilization,National Engineering Research Center for Facilitated Marine Aquaculture,Marine Science and Technology College,Zhejiang Ocean University,Zhoushan 316022,China;School of Fishery,Zhejiang Ocean University,Zhoushan 316022,China

中国海洋大学学报（自然科学英文版）

[1]ZHANG Ying;MENG Lei;MIAO Zengliang;WEI Liming;LIU Bingjian;LIU Liqin;GONG Li;Lü Zhenming-.Complete Mitochondrial Genome of Myra affinis(Decapoda:Brachyura:Leucosiidae)and Its Phylogenetic Implications for Brachyura)[J].中国海洋大学学报（自然科学英文版）,2022(04):987-997

Myra,Leucosiidae,mitoge,Matutidae,Eubrachyura,Raninoida

Complete,Mitochondrial,Genome,affinis,Decapoda,Brachyura,Its,Phylogenetic,Implications,Knowledge,mitochondrial,mitogenomes,can,help,molecular,evolution,relationships,various,species,To,date,phylogenetic,status,within,remains,unresolved,cause,limited,number,available,In,present,study,was,sequenced,using,next,generation,sequencing,supplement,information,bp,long,includes,protein,coding,genes,PCGs,transfer,tRNAs,ribosomal,rRNAs,putative,control,gion,overall,composition,high,bias,All,start,standard,ATN,codon,stop,TAN,incomplete,Except,Ser,other,have,typical,clover,leaf,secondary,structure,analyses,maximum,likelihood,Bayesian,inference,methods,are,employed,generate,identical,topologies,thereby,supporting,sister,between,first,monophyly,well,established,its,strongly,supported,results,this,work,will,not,only,achieve,better,understanding,characteristics,but,also,provide,relevant,further,studies,phylogeny

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