Photosynthesis involves a series of redox re-actions and is the major source of reactive oxygen species in plant cells. Fluctuating light (FL) levels, which occur commonly in natural environments, affect photosynthesis; however, little is known about the specific effects of FL on the redox reg-ulation of photosynthesis. Here, we performed global quantitative mapping of the Arabidopsis thaliana cysteine thiol redox proteome under constant light and FL conditions. We identified 8857 redox-switched thiols in 4350 proteins, and 1501 proteins that are differentially modified de-pending on light conditions. Notably, proteins re-lated to photosynthesis, especially photosystem I (PSI), are operational thiol-switching hotspots. Exposure of wild-type A. thaliana to FL resulted in decreased PSI abundance, stability, and activity. Interestingly, in response to PSI photodamage, more of the PSI assembly factor PSA3 dynamically switches to the reduced state. Furthermore, the Cys199 and Cys200 sites in PSA3 are necessary for its full function. Moreover, thioredoxin m (Trx m) proteins play roles in redox switching of PSA3, and are required for PSI activity and photosyn-thesis. This study thus reveals a mechanism for redox-based regulation of PSI under FL, and pro-vides insight into the dynamic acclimation of photosynthesis in a changing environment.

Qi Chen;Yixian Xiao;Yu Ming;Rong Peng;Jiliang Hu;Hong-Bin Wang;Hong-Lei Jin

Institute of Medical Plant Physiology and Ecology,School of Pharmaceutical Sciences,Guangzhou University of Chinese Medicine,Guangzhou 510006,China

植物学报（英文版）

[1]Qi Chen;Yixian Xiao;Yu Ming;Rong Peng;Jiliang Hu;Hong-Bin Wang;Hong-Lei Jin-.Quantitative proteomics reveals redox-based functional regulation of photosynthesis under fluctuating light in plants OO)[J].植物学报（英文版）,2022(11):2168-2186

thiols,PSA3,Cys199,Cys200

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