Autophagy is an evolutionarily conserved degrada-tion pathway in eukaryotes;it plays a critical role in nutritional stress tolerance. The circadian clock is an endogenous timekeeping system that generates bi-ological rhythms to adapt to daily changes in the environment. Accumulating evidence indicates that the circadian clock and autophagy are intimately in-terwoven in animals. However, the role of the circa-dian clock in regulating autophagy has been poorly elucidated in plants. Here, we show that autophagy exhibits a robust circadian rhythm in both light/dark cycle (LD) and in constant light (LL) in Arabidopsis. However, autophagy rhythm showed a different pat-tern with a phase-advance shift and a lower ampli-tude in LL compared to LD. Moreover, mutation of the transcription factor LUX ARRHYTHMO (LUX) re-moved autophagy rhythm in LL and led to an en-hanced amplitude in LD. LUX represses expression of the core autophagy genes ATG2, ATG8a, and ATG11 by directly binding to their promoters. Phe-notypic analysis revealed that LUX is responsible for improved resistance of plants to carbon starvation, which is dependent on moderate autophagy activity. Comprehensive transcriptomic analysis revealed that the autophagy rhythm is ubiquitous in plants. Taken together, our findings demonstrate that the LUX-mediated circadian clock regulates plant autophagy rhythms.

Ming-Kang Yang;Xiao-Jie Zhu;Chu-Min Chen;Xu Guo;Shu-Xuan Xu;Ya-Rou Xu;Shen-Xiu Du;Shi Xiao;Bernd Mueller-Roeber;Wei Huang;Liang Chen

State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources,College of Life Sciences,South China Agricultural University,Guangzhou 510642,China;Guangdong Laboratory for Lingnan Modern Agriculture,Guangzhou 510642,China;Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms,South China Agricultural University,Guangzhou 510642,China;State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources,School of Life Sciences,Sun Yat-Sen University,Guangzhou 510275,China;Department of Molecular Biology,Institute of Biochemistry and Biology,University of Potsdam,14476 Potsdam-Golm,Germany;Max Planck Institute of Molecular Plant Physiology,14476 Potsdam-Golm,Germany

植物学报（英文版）

[1]Ming-Kang Yang;Xiao-Jie Zhu;Chu-Min Chen;Xu Guo;Shu-Xuan Xu;Ya-Rou Xu;Shen-Xiu Du;Shi Xiao;Bernd Mueller-Roeber;Wei Huang;Liang Chen-.The plant circadian clock regulates autophagy rhythm through transcription factor LUX ARRHYTHMO OO)[J].植物学报（英文版）,2022(11):2135-2149

LUX,ARRHYTHMO,timekeeping,terwoven,circa,ATG2,ATG8a,ATG11,notypic

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