典型文献
Base editing-mediated targeted evolution of ACCase for herbicide-resistant rice mutants FA
文献摘要:
Base editing technologies enable precise base alterationsin a target gene without inducing double–stranded breaks, and thus are powerful for targeted gene evolution in vivo (Zhan et al., 2021). The newly evolved cytosine deami-nases such as evoAPOBEC1, evoFERNY and evoCDA1, adenine deaminase TadA8e, and a near-protospacer ad-jacent motif (PAM)less SpRYCas9 variant, have greatly in-creased base editing efficiency and expanded the editing window in plants (Zhang et al., 2021; Xu et al., 2021c; Tan et al., 2022). Dual cytosine and adenine editors enable con-current C-to-T and A-to-G conversions and thus enrich the editing outcome. Although plant dual editors have been de-veloped (Li et al., 2020;Xu et al., 2021a), they are rarely ap-plied in evolving plant genes due to low efficiencies.
文献关键词:
中图分类号:
作者姓名:
Hongzhi Wang;Yuxin He;Yingying Wang;Zuren Li;Jiannan Hao;Yijiao Song;Mugui Wang;Jian-Kang Zhu
作者机构:
Shanghai Center for Plant Stress Biology and Center for Excellence in Molecular Plant Sciences,Chinese Academy of Sciences,Shanghai 201602,China;University of Chinese Academy of Sciences,Beijing 100049,China;Hunan Provincial Key Laboratory for Biology and Control of Weeds,Hunan Academy of Agricultural Sciences,Changsha 410125,China;College of Life Sciences,Nanjing Agricultural University, Nanjing 210095,China;Institute of Advanced Biotechnology and School of Life Sciences,Southern University of Science and Technology, Shenzhen 518055,China;Center for Advanced Bioindustry Technologies,Chinese Academy of Agricultural Sciences,Beijing 100081,China
文献出处:
引用格式:
[1]Hongzhi Wang;Yuxin He;Yingying Wang;Zuren Li;Jiannan Hao;Yijiao Song;Mugui Wang;Jian-Kang Zhu-.Base editing-mediated targeted evolution of ACCase for herbicide-resistant rice mutants FA)[J].植物学报(英文版),2022(11):2029-2032
A类:
alterationsin,deami,nases,evoAPOBEC1,evoFERNY,evoCDA1,TadA8e,SpRYCas9,2021c
B类:
Base,editing,mediated,targeted,evolution,ACCase,herbicide,resistant,rice,mutants,FA,technologies,enable,precise,base,without,inducing,double,stranded,breaks,thus,powerful,vivo,newly,evolved,cytosine,such,adenine,deaminase,near,protospacer,jacent,motif,PAM,less,variant,have,greatly,creased,efficiency,expanded,window,plants,Zhang,Xu,Tan,Dual,editors,current,conversions,enrich,outcome,Although,dual,been,veloped,Li,2021a,they,rarely,ap,plied,evolving,genes,due,low,efficiencies
AB值:
0.528815
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