典型文献
An in planta haploid induction system in Brassica napusFA
文献摘要：
Doubled haploid technology is widely used to accelerate plant breeding, but its use in the im-portant oilseed crop Brassica napus L. is limited because B. napus haploids could only be ob-tained through in vitro anther or microspore cultures. Recently, maize (Zea mays) lines containing mutations in Domain of unknown function 679 membrane protein (DMP) were used as haploid inducer lines. This new haploid induction mechanism has been extended to several other plants, including the dicots Arabidopsis thaliana, tomato (Solanum lyco-persicum), and tobacco (Nicotiana tabacum). Here, we knocked out four BnaDMP genes in the B. napus cultivar Westar using a clustered reg-ularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 vector with an enhanced green fluorescent protein ex-pression cassette. Plants with DMP mutations in B. napus in the T0, T1, and T2 generations ex-hibited a haploid induction rate up to 2.53％. These results suggest that targeting BnaDMP could be useful for haploid induction in B. napus.
文献关键词：
中图分类号：
作者姓名：
Yifan Li;Dan Li;Qing Xiao;Huadong Wang;Jing Wen;Jinxing Tu;Jinxiong Shen;Tingdong Fu;Bin Yi
作者机构：
National Key Laboratory of Crop Genetic Improvement,National Center of Rapeseed Improvement,Huazhong Agricultural University,Wuhan 430070,China;Key Laboratory of Crop Physiology,Ecology and Genetic Breeding,Ministry of Education,Jiangxi Agricultural University,Nanchang 330045,China
文献出处：
引用格式：
[1]Yifan Li;Dan Li;Qing Xiao;Huadong Wang;Jing Wen;Jinxing Tu;Jinxiong Shen;Tingdong Fu;Bin Yi-.An in planta haploid induction system in Brassica napusFA)[J].植物学报（英文版）,2022(06):1140-1144
A类：
napusFA,dicots,lyco,BnaDMP
B类：
An,planta,induction,system,Brassica,Doubled,technology,widely,used,accelerate,breeding,but,its,portant,oilseed,crop,limited,because,haploids,could,only,tained,through,vitro,anther,microspore,cultures,Recently,maize,Zea,mays,lines,containing,mutations,Domain,unknown,function,membrane,protein,were,inducer,This,new,mechanism,has,been,extended,several,other,plants,including,Arabidopsis,thaliana,tomato,Solanum,persicum,tobacco,Nicotiana,tabacum,Here,knocked,out,four,genes,cultivar,Westar,using,clustered,reg,ularly,interspaced,short,palindromic,repeats,CRISPR,associated,vector,enhanced,green,fluorescent,pression,cassette,Plants,T0,generations,hibited,up,These,results,suggest,that,targeting,useful
AB值：
0.615667
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