典型文献
Delayed callose degradation restores the fertility of multiple P/TGMS lines in Arabidopsis
文献摘要：
Photoperiod/temperature-sensitive genic male ster-ility (P/TGMS) is widely applied for improving crop production. Previous investigations using the rever-sible male sterile (rvms) mutant showed that slow development is a general mechanism for restoring fertility to P/TGMS lines in Arabidopsis. In this work, we isolated a restorer of rvms–2 (res3), as the male sterility of rvms–2 was rescued by res3. Phenotype analysis and molecular cloning show that a point mutation in UPEX1 l in res3 leads to delayed secretion of callase A6 from the tapetum to the locule and tetrad callose wall degradation. Electro-phoretic mobility shift assay and chromatin immunoprecipitation analysis demonstrated that the tapetal transcription factor ABORTED MICRO-SPORES directly regulates UPEX1 expression, revealing a pathway for tapetum secretory function. Early degradation of the callose wall in the trans-genic line eliminated the fertility restoration effect of res3. The fertility of multiple known P/TGMS lines with pollen wall defects was also restored by res3. We propose that the remnant callose wall may broadly compensate for the pollen wall defects of P/TGMS lines by providing protection for pollen formation. A cellular mechanism is proposed to explain how slow development restores the fertility of P/TGMS lines in Arabidopsis.
文献关键词：
中图分类号：
作者姓名：
Kai-Qi Wang;Ya-Hui Yu;Xin-Lei Jia;Si-Da Zhou;Fang Zhang;Xin Zhao;Ming-Yue Zhai;Yi Gong;Jie-Yang Lu;Yuyi Guo;Nai-Ying Yang;Shui Wang;Xiao-Feng Xu;Zhong-Nan Yang
作者机构：
College of Biological and Environmental Engineering,Jingdezhen University,Jiangxi 333000,China;Shanghai Key Laboratory of Plant Molecular Sciences,College of Life Sciences,Shanghai Normal University,Shanghai 200234,China
文献出处：
引用格式：
[1]Kai-Qi Wang;Ya-Hui Yu;Xin-Lei Jia;Si-Da Zhou;Fang Zhang;Xin Zhao;Ming-Yue Zhai;Yi Gong;Jie-Yang Lu;Yuyi Guo;Nai-Ying Yang;Shui Wang;Xiao-Feng Xu;Zhong-Nan Yang-.Delayed callose degradation restores the fertility of multiple P/TGMS lines in Arabidopsis)[J].植物学报（英文版）,2022(03):717-730
A类：
rvms,res3,UPEX1,callase,tapetum,locule,tetrad,phoretic,tapetal,ABORTED,MICRO,SPORES
B类：
Delayed,callose,degradation,restores,fertility,multiple,TGMS,lines,Arabidopsis,Photoperiod,temperature,sensitive,genic,male,widely,applied,improving,crop,production,Previous,investigations,using,rever,sible,sterile,mutant,showed,that,slow,development,general,mechanism,restoring,In,this,work,isolated,restorer,sterility,was,rescued,by,Phenotype,analysis,molecular,cloning,point,mutation,leads,delayed,secretion,A6,from,wall,Electro,mobility,shift,assay,chromatin,immunoprecipitation,demonstrated,transcription,directly,regulates,expression,revealing,pathway,secretory,function,Early,eliminated,restoration,effect,known,pollen,defects,also,restored,We,remnant,may,broadly,compensate,providing,protection,formation,cellular,proposed,explain
AB值：
0.441334
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机标中图分类号，由域田数据科技根据网络公开资料自动分析生成，仅供学习研究参考。
