典型文献
MicroRNA is a potential target for therapies to improve the physiological function of skeletal muscle after trauma
文献摘要：
MicroRNAs can regulate the function of ion channels in many organs. Based on our previous study we propose that miR-142a-39, which is highly expressed in denervated skeletal muscle, might affect cell excitability through similar mechanisms. In this study, we overexpressed or knocked down miR-142a-3p in C2C12 cells using a lentivirus method. After 7 days of differentiation culture, whole-cell currents were recorded. The results showed that overexpression of miR-142a-3p reduced the cell membrane capacitance, increased potassium current density and decreased calcium current density. Knockdown of miR-142a-3p reduced sodium ion channel current density. The results showed that change in miR-142a-3p expression affected the ion channel currents in C2C12 cells, suggesting its possible roles in muscle cell electrophysiology. This study was approved by the Animal Ethics Committee of Peking University in July 2020 (approval No. LA2017128).
文献关键词：
中图分类号：
作者姓名：
Xin-Yi Gu;Bo Jin;Zhi-Dan Qi;Xiao-Feng Yin
作者机构：
Department of Orthopedics and Traumatology,Peking University People's Hospital,Beijing,China;Key Laboratory of Trauma and Neural Regeneration(Peking University),Beijing,China;Department of Orthopedics,The First Affiliated Hospital of Nanjing Medical University,Jiangsu Province Hospital,Nanjing,Jiangsu Province,China
文献出处：
引用格式：
[1]Xin-Yi Gu;Bo Jin;Zhi-Dan Qi;Xiao-Feng Yin-.MicroRNA is a potential target for therapies to improve the physiological function of skeletal muscle after trauma)[J].中国神经再生研究（英文版）,2022(07):1617-1622
A类：
142a,denervated,LA2017128
B类：
potential,target,therapies,improve,physiological,function,skeletal,muscle,after,trauma,MicroRNAs,can,regulate,channels,many,organs,Based,our,previous,study,propose,that,miR,which,highly,might,excitability,through,similar,mechanisms,In,this,overexpressed,knocked,3p,C2C12,cells,using,lentivirus,method,After,days,differentiation,culture,whole,currents,were,recorded,results,showed,overexpression,reduced,membrane,capacitance,increased,potassium,density,decreased,calcium,Knockdown,sodium,change,affected,suggesting,its,possible,roles,electrophysiology,This,was,approved,by,Animal,Ethics,Committee,Peking,University,July,approval,No
AB值：
0.566513
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