典型文献
HOTTIP downregulation reduces neuronal damage and microglial activation in Parkinson's disease cell and mouse models
文献摘要：
HOXA transcript at the distal tip (HOTTIP), a newly identified long noncoding RNA, has been shown to exhibit anti-inflammatory effects and inhibit oxygen-glucose deprivation-induced neuronal apoptosis. However, its role in Parkinson's disease (PD) remains unclear. 1-Methyl-4-phenylpyridium (MPP+) and 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) were used to establish PD models in SH-SY5Y and BV2 cells and in C57BL/6 male mice, respectively. In vitro, after HOTTIP knockdown by sh-HOTTIP transfection, HOTTIP and FOXO1 overexpression promoted SH-SY5Y apoptosis, BV2 microglial activation, proinflammatory cytokine expression, and nuclear factor kappa-B and NACHT, LRR and PYD domains-containing protein 3 inflammasome activation. Overexpression of miR-615-3p inhibited MPP+-induced neuronal apoptosis and microglial inflammation and ameliorated HOTTIP- and FOXO1-mediated nerve injury and inflammation. In vivo, HOTTIP knockdown alleviated motor dysfunction in PD mice and reduced neuronal apoptosis and microglial activation in the substantia nigra. These findings suggest that inhibition of HOTTIP mitigates neuronal apoptosis and microglial activation in PD models by modulating miR-615-3p/FOXO1. This study was approved by the Ethics Review Committee of the Affiliated Hospital of Qingdao University, China (approval No. UDX-2018-042) in June 2018.
文献关键词：
中图分类号：
作者姓名：
Peng Lun
作者机构：
Department of Neurosurgery,the Affiliated Hospital of Qingdao University,Qingdao,Shandong Province,China;Department of Neurosurgery,Laiyang People's Hospital,Yantai,Shandong Province,China;Department of Endocrine and Metabolic Diseases,the Affiliated Hospital of Qingdao University,Qingdao,Shandong Province,China;Emergency Department,the Affiliated Hospital of Qingdao University,Qingdao,Shandong Province,China
文献出处：
引用格式：
[1]Peng Lun-.HOTTIP downregulation reduces neuronal damage and microglial activation in Parkinson's disease cell and mouse models)[J].中国神经再生研究（英文版）,2022(04):887-897
A类：
phenylpyridium,UDX
B类：
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AB值：
0.592546
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