典型文献
β-1,2-Mannan-based glycoconjugates as potential antifungal vaccines
文献摘要：
Phosphorylated di-,tri-and tetra-saccharides of β-1,2-mannan antigen derived from Candida albicans(C.albicans)cell wall were synthesized and covalently conjugated with keyhole limpet hemocyanin(KLH)and human serum albumin(HSA)via a bifunctional linker under mild conditions.The semi-synthetic β-1,2-mannoside-KLH conjugates were evaluated for the immunization of BALB/c mice.The ELISA results revealed that all three conjugates could elicit high levels of specific IgG antibodies and the acquired anti-sera could effectively identify the β-1,2-mannan epitope.Furthermore,the immunofluorescence and flow cytometry assays also uncovered that the induced antibodies,especially that obtained from immuniza-tion with β-1,2-mannotriose-KLH conjugate(1b),could bind well to fungi cell.Eventually,the structure-immunogenicity relationship analysis of β-mannan showed that the length of oligo-β-mannoses had a big impact on their immunogenicity and β-1,2-mannotriose showed the strongest immunogenicity.The results suggested the great potential of β-1,2-mannotriose-KLH conjugate as an antifungal vaccine candi-date.
文献关键词：
中图分类号：
作者姓名：
Jun Liao;Bo Pan;Xiaobin Zhuo;Guochao Liao;Yun Gao;Zhenzhen Yao;Lianghua Wang;Qiuye Wu;Weihua Pan;Binghua Jiao;Qingjie Zhao
作者机构：
Department of Biochemistry and Molecular Biology,College of Basic Medical Sciences,Second Military Medical University,Shanghai 200433,China;School of Pharmacy,Second Military Medical University,Shanghai 200433,China;Department of Dermatology,Changzheng Hospital,Second Military Medical University,Shanghai 200003,China;International Institute for Translational Chinese Medicine,Guangzhou University of Chinese Medicine,Guangzhou 510006,China
文献出处：
引用格式：
[1]Jun Liao;Bo Pan;Xiaobin Zhuo;Guochao Liao;Yun Gao;Zhenzhen Yao;Lianghua Wang;Qiuye Wu;Weihua Pan;Binghua Jiao;Qingjie Zhao-.β-1,2-Mannan-based glycoconjugates as potential antifungal vaccines)[J].中国化学快报（英文版）,2022(09):4345-4349
A类：
Mannan,glycoconjugates,limpet,hemocyanin,mannoside,immuniza,mannotriose,mannoses
B类：
potential,antifungal,vaccines,Phosphorylated,tetra,saccharides,mannan,antigen,derived,from,Candida,albicans,cell,wall,were,synthesized,covalently,conjugated,keyhole,KLH,human,serum,albumin,HSA,via,bifunctional,linker,under,mild,conditions,semi,synthetic,evaluated,immunization,BALB,mice,results,revealed,that,three,could,elicit,high,levels,specific,IgG,antibodies,acquired,sera,effectively,identify,epitope,Furthermore,immunofluorescence,flow,cytometry,assays,also,uncovered,induced,especially,obtained,1b,bind,well,fungi,Eventually,structure,immunogenicity,relationship,analysis,showed,length,oligo,had,big,impact,their,strongest,suggested,great,candi,date
AB值：
0.551977
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