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典型文献
High-performance cascade nanoreactor based on halloysite nanotubes-integrated enzyme-nanozyme microsystem
文献摘要:
Various enzymatic reactions or enzymatic cascade reactions occur efficiently in biological microsystems due to space constraints or orderly transfer of intermediate products.Inspired by this,the horseradish peroxidase(HRP)-like nanozyme(Fe-aminoclay)was in situ synthesized on the surface of alkali-activated halloysite nanotubes and the natural enzyme(glucose oxidase,GOx)was immobilized on it to construct a high-efficiency GOx-FeAC@AHNTs cascade nanoreactor.In which,FeAC@AHNTs can not only be used as a carrier for immobilized enzymes,but also help its catalytic activity to cooperate with glucose oxidase in a cascade reaction.The microcompartments and substrate channel effect of this enzyme-nanozyme microsystem exhibit a superior catalytic performance than that of natural enzyme system,and exhibits excellent long-term stability and recyclability.Subsequently,the GOx-FeAC@AHNTs cascade nanoreactor was employed as a glucose colorimetric platform,which displayed a low detection limit(0.47 μmol/L)in glucose detection.This enzyme-nanoenzyme nanoreactor provides a simple and effective example for constructing a multi-enzyme system with limited space,and lays the foundation for subsequent research in the fields of biological analysis and catalysis.
文献关键词:
作者姓名:
Yan Liu;Rui Lv;Shiyong Sun;Daoyong Tan;Faqin Dong;Yevgeny A.Golubev;Xiaoqin Nie;Olga B.Kotova;Jin Liu;Ke Wang
作者机构:
School of Environment and Resource,Key Laboratory of Solid Waste Treatment and Resource Recycle of Ministry of Education,Southwest University of Science and Technology,Mianyang 621010,China;Yushkin's Institute of Geology,Komi Science Center,Ural Branch of RAS,ul.Pervomayskaya,54,Syktyvkar 167982,Russia
引用格式:
[1]Yan Liu;Rui Lv;Shiyong Sun;Daoyong Tan;Faqin Dong;Yevgeny A.Golubev;Xiaoqin Nie;Olga B.Kotova;Jin Liu;Ke Wang-.High-performance cascade nanoreactor based on halloysite nanotubes-integrated enzyme-nanozyme microsystem)[J].中国化学快报(英文版),2022(02):807-811
A类:
microsystem,aminoclay,FeAC,AHNTs,microcompartments,nanoenzyme
B类:
High,performance,cascade,nanoreactor,halloysite,nanotubes,integrated,nanozyme,Various,enzymatic,reactions,occur,efficiently,biological,microsystems,due,space,constraints,orderly,transfer,intermediate,products,Inspired,by,this,horseradish,peroxidase,HRP,like,was,situ,synthesized,surface,alkali,activated,natural,glucose,GOx,immobilized,high,efficiency,which,can,only,used,carrier,enzymes,but,also,help,catalytic,activity,cooperate,substrate,channel,superior,than,that,exhibits,excellent,long,stability,recyclability,Subsequently,employed,colorimetric,platform,displayed,low,detection,This,provides,simple,effective,example,constructing,multi,limited,lays,foundation,subsequent,research,fields,analysis,catalysis
AB值:
0.473385
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